为了研究壳聚糖（Chitosan，CS）/普鲁兰多糖（Pullulan，LP）/聚乙烯醇（Polyvinyl Alcohol，PVA）/胶原蛋白（Collagen，GL）食品包装膜理化性质及结构特征，该研究以CS、LP、PVA和GL为原料，按一定比例混合，采用流延成膜法制备多元复合膜，测定复合膜的机械性能、水溶性、溶胀率、色度和透光率等指标，并利用红外光谱、X衍射光谱和扫描电镜表征膜的结构。结果表明：CS、LP和CS-LP膜材分别添加到PVA-GL中，复合膜厚度逐渐增加，拉伸强度逐渐降低，而断裂伸长率呈递增的趋势，其中四元膜厚度为142.50 μm，抗拉强度为11.21 MPa，断裂伸长率为169.24%；提高复合膜的膨胀率，降低了复合膜的溶解度，四元膜的膨胀率最高，溶解度最低，分别为368.16%，58.64%；色度及不透明度的测量发现，四元膜亮度较亮和色差小的特点，且该膜不透明度最小，为2.28 UA/mm；测量其波长在200~800 nm范围内紫外可见光的透光率发现，该膜具有良好的紫外线阻隔能力；红外光谱、X衍射光谱及扫描电镜分析结果显示，CS和LP能与PVA-GL相互作用，降低CS和LP的结晶度，形成结构致密的均相体系。研究结果为CS/PL/PVA/GL食品包装膜的进一步研究提供了可以借鉴的科学依据。

The physicochemical properties and structural characteristics of chitosan (CS)/pullulan (LP)/polyvinyl alcohol (PVA)/collagen (GL) food packaging films were studied using CS, LP, PVA, and GL as raw materials and mixed at certain proportions. A multi-component composite film was prepared using the film casting method, and the mechanical properties, water solubility, swelling rate, chromaticity, and light transmittance of the composite film were measured. The structure of the film was characterized via infrared spectroscopy, X-ray diffraction spectroscopy, and scanning electron microscopy. When CS, LP, and CS-LP membranes were added to PVA-GL in that order, the thickness and elongation at break of the composite film increased gradually but tensile strength decreased gradually. The thickness of the quaternary membrane was 142.50 μm, its tensile strength was 11.21 MPa, and elongation at break was 169.24%. The expansion rate of the composite film was increased but its solubility was reduced. The expansion rate of the quaternary film was the highest and its solubility was the lowest, at 368.16% and 58.64%, respectively. The measurements of chromaticity and opacity revealed that the quaternary film was brighter and showed a smaller color difference, with the lowest opacity of 2.28 UA/mm. The transmittance of UV and visible light in the wavelength range of 200~800 nm was measured, and the film showed excellent UV-blocking ability. Finally, infrared spectroscopy, X-ray diffraction spectroscopy, and scanning electron microscopy revealed that CS and LP interacted with PVA-GL, reduced the crystallinity of CS and LP, and formed a dense homogeneous system. These findings provide a scientific basis for further research on CS/PL/PVA/GL food packaging films.

国家自然科学基金面上项目（31972021）；岭南重点实验室项目（NZ2021033）；广东省重点研发计划项目（2019B020212003；2021B0202060001）；广东省现代农业产业技术体系创新团队建设专项（2022KJ117）；佛山市科技项目（基塘农业关键技术研究与示范,多源光谱图像信息融合的草鱼生鱼片快速智能检测技术研发与应用）；广东省农业科学院水产中心建设项目