多穗柯叶醇提物改善Ⅱ型糖尿病小鼠糖脂代谢及氧化应激的作用

Improving Glycolipid Metabolism and Oxidative Stress in Type Ⅱ Diabetic Mice via Leaf Ethanolic Extracts of Lithocarpus polystachyus Rehd.

摘要:

研究多穗柯叶醇提物（LEL）对2型糖尿病模型小鼠糖脂代谢及氧化应激反应的改善作用。将成模的2型糖尿病（T2DM）小鼠模型，分为空白对照组、模型对照组、阳性对照组及LEL高、低剂量（30、15 g/kg）组，连续给药4周。实验期间观察小鼠的毛色、精神状态并测定空腹血糖（FBG）。实验结束后，检测小鼠口服糖耐量及血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）水平，检测肝组织中肝糖原、过氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、丙二醛（MDA）水平，并进行肝组织病理切片检查。结果显示，LEL可改善T2DM小鼠的精神状态及多尿症状。低、高剂量组较模型对照组空腹血糖分别降低了45.38%、46.25%，血清OGTT、TG、TC、LDL-C水平显著降低，HDL-C显著升高（P<0.01）；肝组织中肝糖原含量及SOD、GSH活性显著升高，MDA含量显著降低（P<0.01），脂肪变性及炎性细胞浸润程度明显减轻。结果表明，LEL具有明显降低T2DM小鼠血糖，升高肝糖原含量、改善脂质代谢与降低氧化应激损伤作用。

Abstract:

The improvement effects of leaf extracts of Lithocarpus polystachyus Rehd. on glycolipid metabolism and reduce oxidative stress in mice with Type Ⅱ diabetes was examined. Type Ⅱ diabetes mellitus (T2DM) mice were divided into the following groups: blank control group, model control group, positive control group, LEL high and low-dose (30, 15 g/kg) groups, and mice of each group were continuously administered the corresponding drugs for 4 weeks. During the experiment, coat color, mental state, body weight, and fasting blood glucose (FBG) levels of the mice were measured. After the experiment, the mice were tested for oral glucose tolerance and serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels. Histopathological examination of liver tissue was conducted at the end of the experiment and glycogen, superoxide dismutase (SOD), reduced glutathione (GSH), and malondialdehyde (MDA) levels were measured. The results showed that LEL could improve the mental state and polyuria of T2DM mice. Compared with the model control group, the fasting blood glucose in low- and high-dose LEL groups decreased by 45.38% and 46.25% respectively, and the serum levels of OGTT, TG, TC, and LDL-C in were also significantly reduced, whereas the HDL-C level was significantly increased (P<0.01). Liver glycogen content and SOD and GSH activities in liver tissue were significantly high, whereas the MDA content was significantly low (P<0.01), and the steatosis and inflammatory cell infiltration in T2DM were significantly alleviated after administering LEL for 4 weeks. The results indicate that LEL can significantly reduce blood glucose, increase liver glycogen content, improve lipid metabolism, and reduce oxidative stress damage in T2DM mice.