研究蒜氨酸对棕榈酸诱导的HepG2细胞脂质积聚的影响，并探讨蒜氨酸的作用机制与自噬的关系。利用CCK8法测定细胞活性，采用尼罗红染色法观察细胞内脂质堆积情况，并使用ELISA试剂盒法检测甘油三酯和总胆固醇含量，采用Western Blot测定自噬标志蛋白含量。结果显示，与空白组相比，阳性对照组中HepG2细胞的总胆固醇和甘油三酯含量显著增加，分别增加约1.69和1.36倍；而在蒜氨酸在40~160 µmol/L浓度范围作用下，细胞脂滴减少，总胆固醇和甘油三酯显著降低，且呈现剂量依赖性，在160 µmol/L浓度的蒜氨酸作用下总胆固醇和甘油三酯含量均极显著下降，分别降低了37.52%和33.33%。同时，蒜氨酸在80~160 µmol/L浓度范围内显著增加自噬标志蛋白LC3-Ⅱ/LC3-Ⅰ比例，分别增加了23.87%和66.13%。因此得出，蒜氨酸改善了棕榈酸诱导的HepG2细胞脂质积聚，并可能通过激活细胞自噬的活性起到改善细胞脂质积聚的作用。

The effect of alliin on palmitic acid-induced lipid accumulation in HepG2 cells was studied, and the relationship between lipid metabolism and autophagy was also explored in the article. The cell activity was measured by CCK8 method, the intracellular lipid accumulation was observed by Nile red staining, the contents of triglyceride and total cholesterol were detected by ELISA kits, and the content of autophagy marker protein was determined by Western blot. The results showed that compared with the blank group, the contents of total cholesterol and triglyceride in HepG2 cells in the positive control group increased significantly, about 1.69-times and 1.36-times respectively; Under the action of alliin in the concentration range of 40~160 µmol/L, the cell lipid droplets decreased, and the contents of total cholesterol and triglyceride decreased significantly in a dose-dependent manner. Under the action of allicin in the concentration range of 160 µmol/L, the contents of total cholesterol and triglyceride decreased significantly, 37.52% and 33.33% respectively. Meanwhile, alliin significantly increased the ratio of autophagy marker protein LC3-Ⅱ/LC3-Ⅰ in the concentration range of 80~160 µmol/L, increasing by 23.87% and 66.13% respectively. Therefore, alliin improved the lipid metabolism disorder in HepG2 cells induced by PA (palmitic acid), and may ameliorate the lipid accumulation by activating the activity of autophagy.

吉林省科技发展计划项目（YDZJ202301ZYTS162；YDZJ202201ZYTS401）；川菜发展研究中心科研项目（CC21Z01）；吉林农业科技学院营养与健康食品科研团队经费资助