醋醅源潜在益生菌的筛选及其对秀丽隐杆线虫寿命延长作用

Screening of Potential Probiotics Derived from Cupei and Their Effect on Prolonging the Lifespan of Caenorhabditis elegans

摘要:

该研究对从传统醋醅中分离的7株菌株进行体外益生功能检测，并研究其对秀丽隐杆线虫（Caenorhabditis elegans，C. elegans）寿命的影响。7株菌通过溶血性、抑菌能力、产蛋白酶、自聚集和共聚集、疏水性以及DPPH自由基清除能力检测有明显差异，筛选出两株斯塔贝基斯鲁梅利杆菌（Rummeliibacillus stabekisii，R. stabekisii）CY2与CY4具有无溶血性，产蛋白酶含量高（11.49 mm和13.24 mm），24 h自聚集与共聚集能力强，24 h细胞表面疏水性突出，DPPH自由基清除能力较好（上清液48.69%和59.22%，菌体44.00%和41.20%），进一步耐酸性实验得出CY2在pH值3.0时耐酸性显著优于CY4，且能够繁殖生长（4~8 h时存活率升高8.82%），并且CY2具有较高的LPS降解能力（46.95%），秀丽隐杆线虫（C. elegans）寿命实验表明CY2能够显著延长寿命，单独饲喂相较于OP50组，平均寿命延长1.37 d，延长率为15.91%（P<0.05），最大寿命延长1.00 d，与OP50混合饲喂，平均寿命延长1.47 d，延长率为17.10%（P<0.05），最大寿命延长2.00 d。该研究结果表明斯塔贝基斯鲁梅利杆菌（R. stabekisii）CY2益生特性明显，具有较好的开发潜力，同时也为发掘新型食源潜在益生菌提供参考和依据。

Abstract:

Seven strains of bacteria were isolated from traditional Cupei and tested for in vitro probiotic function as well as their effect on the longevity of Caenorhabditis elegans (C. elegans). The strains differed significantly in terms of hemolytic ability, antibacterial activity, protease production, self-aggregation and co-aggregation, hydrophobicity, and DPPH free radical scavenging ability. Two strains of Rummeliibacillus stabekisii, CY2 and CY4, were selected based on no hemolysis and high protease content (11.49 mm and 13.24 mm), strong 24-h self-aggregation and co-aggregation ability, outstanding 24-h cell surface hydrophobicity, and good DPPH free radical scavenging ability (supernatant 48.69% and 59.22%, bacterial cell 44.00% and 41.20%). The results of acid resistance experiments showed that CY2 had significantly better acid resistance at pH value 3.0 than did CY4 and was able to multiply and grow; the survival rate increased by 8.82% at 4~8 h. CY2 also showed a high LPS degradation ability (46.95%). The results of the C. elegans lifespan experiment showed that CY2 could significantly prolong lifespan. Compared with the OP50 group, the average lifespan was prolonged by 1.37 days, the prolongation rate was 15.91% (P<0.05), and the maximum lifespan was extended by 1.00 d. Mixed feeding with OP50 prolonged the average lifespan by 1.47 d, the prolongation rate was 17.10% (P<0.05), and the maximum lifespan was prolonged by 2.00 d. These results indicated that R. stabekisii CY2 has signficant prebiotic properties and good development potential. The results of this study provide a reference and basis for exploring potential probiotics from new food sources.