3种微量化试剂对沙门氏菌及近源菌的生化鉴定结果比较

Comparison of Biochemical Identification Results of Three Identification Kits for Salmonella and Related Bacteria

摘要:

评价本实验室研制的沙门氏菌微量生化鉴定试剂盒（简称EasyID）的使用性能。用49株菌（包括沙门氏菌12株标准株，16株分离株；志贺氏菌8株标准株；柠檬酸杆菌1株标准菌和8株分离株；变形杆菌1株标准株和2株分离株；产气肠杆菌1株标准株），测试EasyID，传统液态生化管（简称HKM）以及国内其他品牌（简称GSA和GSB）的四种鉴定试剂盒。结果表明：49株测试菌株的鉴定结果，EasyID、GSA和GSB三种试剂与HKM传统液态鉴定试剂盒鉴定结果相比总体符合率分别为100%、94.46%、95.10%，EasyID相比GSA和GSB，与HKM的符合率更高；EasyID、HKM、GSA和GSB四种鉴定试剂盒与伯杰氏手册相比总体符合率分别为97.96%、97.96%、93.00%、94.70%，EasyID和HKM符合率最高，其次为GSB，GSA稍低。结论：EasyID沙门氏菌微量生化鉴定试剂盒在可靠性上与传统生化管完全一致，在使用方便性上，采用一步加样技术的EasyID优于HKM、GSA和GSB。

Abstract:

The biochemical identification kit for Salmonella (EasyID) developed in the laboratory was evaluated. A total of 49 bacterial strains (including 12 standard strains and 16 isolates of Salmonella, 8 standard strains of Shigella, 1 standard strain and 8 isolates of Citrobacter, 1 standard strain and 2 isolates of Proteus, and 1 standard strain of Enterobacter aerogenes) were used to test four Salmonella identification kits: EasyID, traditional liquid biochemical tube (HKM), and other domestic brands (GSA and GSB). For the 49 tested strains, the identification results of three kits (EasyID, GSA and GSB) were compared with those of the HKM traditional liquid identification kit, and the overall coincidence rates were 100%, 94.46%, and 95.10% respectively. Compared with GSA and GSB, EasyID had a higher coincidence rate with HKM. Compared with the Bergey’s manual, the four identification kits EasyID, HKM, GSA, and GSB yielded overall coincidence rates of 97.96%, 97.96%, 93.00%, and 94.70%, respectively. EasyID and HKM had the highest coincidence rate, followed by GSB, whereas the coincidence rate of GSA was slightly lower. Conclusion: The reliability of the EasyID Salmonella identification kit was consistent with that of traditional biochemical tubes. Furthermore, EasyID, which uses a one-step sampling technology, was easier to use than HKM, GSA, and GSB.