该实验采用保加利亚乳杆菌、嗜热链球菌、醋酸菌3种菌种发酵猴头菇，通过比较发酵液的DPPH自由基清除率，确定猴头菇发酵的最优条件。用猴头菇益生菌发酵液对酒精性胃溃疡模型的小鼠进行灌胃，测定胃溃疡小鼠胃组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）和血清中炎症因子白介素-6（IL-6）、白介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）含量。结果表明，最佳发酵条件为起始pH值6.5、菌种复配比（保加利亚乳杆菌:嗜热链球菌:醋酸菌）1:1:1、接种量8.8%、发酵温度37.5 ℃、发酵时间27 h，发酵液菌落数为8.72×109 CFU/mL、发酵液的DPPH自由基清除率可达79.59%。与模型组相比，高剂量组小鼠胃组织的SOD和CAT水平分别升高了71.38%和99.89%、MDA含量降低了47.45%；小鼠血清中的炎症因子TNF-α和IL-6含量分别降低了32.66%和34.95%、IL-10含量升高了100.46%，表明猴头菇益生菌发酵液对小鼠的急性酒精性胃溃疡有保护作用。该研究为猴头菇的应用提供了理论支持，为猴头菇新产品的开拓和益生菌的开发提供理论基础。

In this experiment, three strains of Lactobacillus bulgaricus, Streptococcus thermophilus and Acetic acid bacteria were used to fermentat Hericium erinaceus. The DPPH free radical scavenging rates of the fermentation broth samples were compared to determine the optimal conditions for fermenting Hericium hericium. Mice with alcoholic gastric ulcer were subjected to intragastric administration with hericium ordosica probiotic fermentation broth. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) in gastric tissues and contents of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor -α (TNF-α) in the serum of the mice with alcoholic gastric ulcer were measured. The results showed that the optimal fermentation conditions were as follows: initial pH 6.5; bacteria compound ratio 1:1:1 (Lactobacillus delbrueckii subsp. bulgaricus: Streptococcus thermophilus: Acetobacter); inoculation amount 8.8%; fermentation temperature 37.5 ℃; fermentation time 27 h. The colony number of the fermentation broth was 8.72×109 CFU/mL and the DPPH free radical scavenging rate of fermentation broth was 79.59%. Compared with the model group, the SOD and CAT levels increased by 71.38% and 99.89% whilst the MDA content decreased by 47.45% in gastric tissues of mice in high-dose group. The contents of TNF-α and IL-6 in the serum of mice decreased by 32.66% and 34.95%, respectively, and the content of IL-10 increased by 100.46%. The results showed that the probiotics fermentation broth of hericium had a protective effect on acute alcoholic gastric ulcer in mice. This study provides a theoretical support for the application of Hericium hericium, and a theoretical basis for the development of new products of Hericium hericium and probiotics.

黑龙江省“揭榜挂帅”科技攻关项目（2021ZXJ03B01-1）