Abstract:The effects of the established life-prolonging diet on human gut microbiota in Guangxi were investigated in this study. A total of 14 volunteers were screened and required to follow this diet for two weeks with high levels of compliance. Fecal samples were collected before and after the dietary intervention and analyzed using 16S rDNA high-throughput sequencing. The results show that the gut microbiota diversity of volunteers changed in multiple respects after the dietary intervention. At the phylum level, the relative abundance of TM7 significantly decreased (from 0.05% to 0.01%), and the relative abundance of Proteobacteria and the ratio of Firmicutes to Bacteroidetes also decreased. At the genus level, the relative abundance of Coprococcus increased significantly (from 1.04% to 1.39%), while the relative abundance of Escherichia (from 14.00% to 9.62%), Ruminococcus (from 5.67% to 2.48%), and Megamonas (from 4.06% to 0.04%) significantly decreased. Redundancy analysis indicated that changes in the gut microbiota were closely related to daily food consumption, with the average daily intake of vegetables being the most relevant (r2=0.89, p<0.01). The above results prove that the structure of human gut microbiota in Guangxi could be effectively regulated by the life-prolonging diet. Furthermore, the abundance of certain harmful bacteria in the intestinal tract was reduced, which has a positive effect on maintaining a healthy body. These results provide a reference for revealing an effective mechanism for regulating health and longevity through diet in terms of gut microbiota.
