基于OAV和GC-O-MS法鉴定香椿中的关键香气成分

Identification of the Key Aroma Components in Toona sinensis Using Odor Activity Value and Gas Chromatography-olfactometry-mass Spectrometry

摘要:

采用固相微萃取（Solid-Phase Microextraction，SPME）与溶剂辅助风味蒸发（Solvent-Assisted Flavor Evaporative，SAFE）法结合香气活性值（Odor Activity Value，OAV）和气相色谱-嗅闻-质谱联用技术（Gas Chromatography-Olfactory-Mass Spectrometry，GC-O-MS）分析鉴定新鲜香椿中关键香气成分。结果显示，采用两种萃取方法共鉴定出90种挥发性化合物，主要包括含硫类12种，醛类11种，萜烯类34种，酮类2种，醇类4种，酯类4种，酚类1种，酸类1种，烷烃类15种，环烷烃类4种，其他类2种。经OAV值计算，共确定出(E,E)/(E,Z)/(Z,Z)-二丙烯基二硫醚、反-2-巯基-3,4-二甲基-2,3-二氢噻吩、2-甲基环硫乙烷、己醛、3-甲基-正丁醛、反-2-己烯醛、反-2-辛烯醛等9种香气成分；经GC-O-MS分析，共鉴定出(E,E)/(E,Z)/(Z,Z)-二丙烯基二硫醚、2-甲基环硫乙烷、反-2-巯基-3,4-二甲基-2,3-二氢噻吩、己醛、反-2-己烯醛等7种香气活性物质。综合OAV和GC-O-MS分析，最终确认(E,E)/(E,Z)/(Z,Z)-二丙烯基二硫醚、反-2-巯基-3,4-二甲基-2,3-二氢噻吩、2-甲基环硫乙烷、己醛、反-2-己烯醛为新鲜香椿的关键香气成分。

Abstract:

The key aroma components in fresh Toona sinensis were analyzed and identified using solid-phase microextraction and solvent assisted flavor evaporation, in combination with odor activity value (OAV) and gas chromatography olfactory mass spectrometry (GC-O-MS). The two extraction methods identified 90 volatile compounds, including 12 sulfur compounds, 11 aldehydes, 34 terpenes, 2 ketones, 4 alcohols, 4 esters, 1 phenol, 1 acid, 15 alkanes, 4 cycloalkanes, and 2 other compounds. Through OAV calculation, nine aroma active components were identified, including (E,E)-, (E,Z)-, and (Z,Z)-bis(1-propenyl) disulfide, trans-2-mercapto-3,4-dimethyl-2,3-dihydrothiophene, methyl-thiirane, hexanal, 3-methyl-butanal, (E)-2-hexanal, and (E)-2-octenal. Seven aroma active compounds were identified using GC-O-MS, including (E,E)-, (E,Z)-, and (Z,Z)-bis(1-propenyl) disulfide, trans-2-mercapto-3,4-dimethyl-2,3-dihydrothiophene, methyl-thiirane, hexanal, and (E)-2-hexanal. The OAV and GC-O-MS analyses confirmed that (E,E)-, (E,Z)-, and (Z,Z)-bis(1-propenyl) disulfide, trans-2-mercapto-3,4-dimethyl-2,3- dihydrothiophene, methyl-thiirane, hexanal, and (E)-2-hexanal were the key aroma components in fresh Toona sinensis.