藏黄连多糖-硒纳米颗粒的制备、结构表征及体外抗炎活性

Preparation, Structural Characterization, and in Vitro Anti-inflammatory Activity of Coptis teeta Polysaccharide-selenium Nanoparticles

摘要:

该研究通过绿色合成中药多糖-硒纳米颗粒，筛选具有抗氧化、抗炎功能的富硒中药多糖保健食品，满足人们生活水平不断提高的要求和养生保健的愿望。通过藏黄连多糖（CTP）绿色还原亚硒酸钠成功构建藏黄连多糖-硒纳米颗粒（CTP-SeNPs），表征其结构，并探索其体外抗氧化与抗炎活性。结果表明，通过FT-IR和XRD验证该研究中成功构建了CTP-SeNPs；EDS-SEM和DLS显示，CTP-SeNPs呈现厚片状，边缘圆润，硒含量为11.66%，粒径约101.96 nm；体外抗氧化试验显示，在0~10 mg/mL浓度范围内，随CTP-SeNPs浓度的增加，其清除自由基能力逐渐增大，最大清除率均能达到60%以上，且体外抗氧化效果优于CTP组；体外细胞试验显示，在250~500 μg/mL浓度范围内，CTP-SeNPs促进RAW264.7细胞增殖的能力显著优于CTP，在250~1 000 μg/mL浓度范围内，CTP-SeNPs抗炎效果显著高于CTP。综上所述，藏黄连多糖绿色还原亚硒酸钠成功构建的藏黄连多糖-硒纳米颗粒具有抗氧化、促抗炎的活性，可为研发富硒中药多糖保健食品提供理论依据。

Abstract:

The green synthesis of the traditional Chinese medicine, polysaccharide-selenium nanoparticles, was investigated to screen for selenium-rich polysaccharide health foods with antioxidant and anti-inflammatory functions to meet the increasing standards of living and the demand for better health. Coptis teeta-selenium nanoparticles (CTP-SeNPs) were prepared using the green reduction of sodium selenite by CTP and characterized and investigated for their in vitro antioxidant and anti-inflammatory properties. The results of Fourier transform infrared spectrometry and X-ray diffraction revealed that the CTP-SeNPs were successfully constructed. Energy dispersive X-ray spectroscopy-scanning electron microscopy and dynamic light scattering showed that the CTP-SeNPs appeared as thick flakes with rounded edges and had a selenium content of 11.66% and a particle size of about 101.96 nm. In vitro antioxidant assays showed that the free radical-scavenging ability of CTP-SeNPs gradually increased with an increase in the concentration in the range of 0~10 mg/mL, and the maximum scavenging rate was over 60%. The in vitro antioxidant effect of CTP-SeNPs was better than that of the CTP group. In vitro cell assays showed that CTP-SeNPs significantly promoted the proliferation of RAW264.7 cells as compared with CTP in the concentration range of 250~500 μg/mL. The anti-inflammatory activity of CTP-SeNPs in the concentration range of 250~1 000 μg/mL was better than that of CTP. In conclusion, CTP-SeNPs exhibits antioxidant and anti-inflammatory properties that may be potentially useful for the development of selenium-rich traditional Chinese medicine polysaccharide health foods.