为探究鞣花酸对甲醛引起的小鼠肝损伤的保护机制，选取32只8周龄雄性ICR小鼠随机分为对照组（生理盐水），甲醛模型组（333 mmol/L），鞣花酸低剂量组（33 mmol/L）和鞣花酸高剂量组（66 mmol/L）。连续灌胃14 d后，解剖并获取肝脏，用于称重且计算其肝脏指数，观察肝脏的形态学变化，分析肝脏中的丙二醛含量（MDA）和超氧化物歧化酶活性（SOD），并通过荧光定量PCR检测肝脏的细胞因子表达水平。与甲醛模型组相比，鞣花酸给药组的肝脏组织，肝细胞排列规则，肝细胞中空泡减少；低剂量组SOD活性（0.97 U/mg）和SOD1（0.98）、SOD2（0.98）表达水平增加，MDA含量（38.51 nmol/mg）和NF-κB（0.78）、IL-1β（0.63）、IL-6（0.87）、TNF-α（0.85）、Bax（0.70）、Caspase-3（0.96）的表达水平降低，高剂量组SOD活性（1.16 U/mg）和SOD1（1.23）、SOD2（1.11），表达水平增加，MDA含量（43.25 nmol/mg）和NF-κB（0.49）、IL-1β（0.61）、IL-6（0.38）、TNF-α（0.79）、Bax（0.68）、Caspase-3（0.93）的表达水平降低。结果表明，鞣花酸通过其抗氧化，抗炎和抗凋亡的特性对甲醛诱导的小鼠肝损伤具有一定的保护作用。

In order to investigate the protective mechanism of ellagic acid against formaldehyde-induced liver injury in mice, 32 8-week-old male ICR mice were randomly divided into four groups: the control group (Con Group; normal saline), 333 mmol/L formaldehyde model group (FA Group; 333 mmol/L), ellagic acid low-dose group (33 mmol/L), and ellagic acid high-dose group (66 mmol/L). After 2 weeks of continuous gavage, the mice were dissected and the liver were collected for weighing, determining liver indices, observing the morphological changes of the liver, and analyzing the malondialdehyde content (MDA) content and superoxide dismutase (SOD) activity in the liver. The expression levels of cytokines in the liver were detected by fluorescence quantitative PCR. Compared with the formaldehyde model group, the arrangement of hepatocytes was regular, and the number of vacuoles in the hepatocytes decreased in the liver tissues of the ellagic acid administration group. For the low-dose group, the SOD activity (0.97 U/mg) and the SOD1 (0.98) and SOD2 (0.98) expression levels increased, whilst the MDA content (38.51 nmol/mg) and the expression levels of NF-κB (0.78), IL-1β (0.63), IL-6 (0.87), TNF-α (0.85), Bax (0.70) and Caspase-3 (0.96) decreased. For the high-dose group, the SOD activity (1.16 U/mg) and the expression levels of SOD1 (1.23) and SOD2 (1.11) increased, whilst the MDA content (43.25 nmol/mg), and the expression levels of NF-κB (0.49), IL-1β (0.61), IL-6 (0.38), TNF-α (0.79), Bax (0.68) and Caspase-3 (0.93) decreased. The results showed that ellagic acid has a certain protective effect on formaldehyde-induced liver injury in mice through its antioxidant, anti-inflammatory and anti-apoptotic properties.

吉林省教育厅科学技术研究项目（JJKH20200357KJ）