研究奶牛场沙门氏菌分离株的流行特征及抗性分析。从成都市某规模化奶牛场采集样品，对菌株进行分离鉴定和血清型分析，药敏检测，毒力基因、耐药基因和抗消毒剂基因检测，对常用消毒剂MIC值检测，以及有效巴氏灭菌温度分析。该研究共采集样品700份，分离16株沙门氏菌，得到4种血清型（肠炎沙门氏菌6株、甲型副伤寒沙门氏菌6株、鼠伤寒沙门氏菌3株、婴儿沙门氏菌1株）；菌株对头孢他啶、左氧氟沙星、亚胺培南敏感，携带24种耐药基因，检出率最高是tetB（93.75%），其次是aaC4（75.00%）；检出12种毒力基因，sipA检测率最高为62.50%；抗消毒剂基因检出率最高为qacEΔ1（56.25%）。16株沙门氏菌对9种不同消毒剂MIC值普遍高于厂家推荐使用浓度的1~3倍；菌株在全脂乳和脱脂乳中100%杀菌温度分别为64.00 ℃和63.50 ℃。该奶牛场沙门氏菌分离菌株对消毒剂抗性较强，但在64.00 ℃能够对其有效杀灭。建议奶牛场加强沙门氏菌防控，规范消毒剂使用。

Samples were collected from a large dairy farm in Chengdu city to study the epidemic characteristics and resistance of Salmonella isolates. Strains of Salmonella were isolated, identified, and seroptyped. Drug susceptibility, virulence, drug resistance, and disinfectant resistance genes were tested. Minimum inhibitory concentrations (MIC) to commonly used disinfectants and effective sterilization temperatures were also determined. A total of 700 samples were collected; 16 strains of Salmonella and four serotypes were isolated (six strains of Salmonella enteritidis, six strains of S. paratyphoid A, three strains of S. typhimurium, and one strain of S. infantis). All strains were sensitive to ceftazidime, levofloxacin, and imipenem. Twenty four drug resistance genes were detected, and the highest detection rate was noted for tetB (93.75%), followed by that of aaC4 (75.00%). There were 12 virulence genes detected, and the highest detection rate was observed for sipA (62.50%); qacEΔ1 showed the highest detection rate of the disinfectant genes (56.25%). The MIC values of 16 Salmonella strains against nine different disinfectants were generally one to three times higher than the recommended concentrations from the manufacturers. The 100% sterilization temperatures of the strains in whole and skim milk were 64.00 ℃ and 63.50 ℃, respectively. The isolated strains of Salmonella on this dairy farm were resistant to some disinfectants, but could be effectively controlled at 64.00 ℃. It is recommended that dairy farms strengthen the prevention and control of Salmonella and the use of disinfectants should be standardized. Key words:

国家重点研发计划项目（2018YFD0500500）；四川省应用基础项目（2019YJ0261）；西南民族大学中央高校基本科研业务费专项资金资助（2020NTD04）