该实验探究了天麻（Gastrodin elata Bl.）、苦荞（Tartary Buckwheat）醇提物的添加对灰树花（Grifola frondosa）胞外多糖（Exopolysaccharide，EPS）合成过程中磷酸葡萄糖变位酶（Phosphogluconate Mutase，PGM）、UDP-葡萄糖焦磷酸化酶（UDP-glucose Pyrophosphorylase，UGPase）和磷酸葡萄糖异构酶（Phosphogluconate Isomerase，GPI）三种关键酶的影响。并以此为前提，采用动物实验进一步探究灰树花发酵液的抗疲劳作用。结果表明：天麻、苦荞醇提物的复配添加可显著增强PGM和UGPase的活力，降低GPI的活力，与空白组相比PGM和UGPase的活力分别增加了86.98%和69.12%，GPI的活力降低了65.78%。此外，其复配发酵液在抗疲劳作用方面表现最佳，显著高于空白组（p<0.05）和实验组（p<0.05）。主要在提高小鼠游泳力竭时间、肝糖原（Hepatic Glycogen，HG）和乳酸脱氢酶（Lactate Dehydrogenase，LDH）含量，降低乳酸（Lactic Acid，LA）和尿素氮（Blood Urea Nitrogen，BUN）含量几个方面体现。研究表明复配液的添加可显著改变灰树花EPS合成途径中关键酶类的活力，从而提升发酵液中EPS的含量，使其具有良好的抗疲劳作用。

In this study, the effects of the addition of ethanolic extracts of Gastrodin elata Bl. and Tartary buckwheat on the key enzymes, phosphogluconate mutase (PGM), UDP-glucose pyrophosphorylase (UGPase) and phosphogluconate isomerase (GPI) during the synthesis of exopolysaccharide (EPS) from Grifola frondose were investigated. On this premise, animal experiments were conducted to explore further the anti-fatigue effect of Grifola frondosa-derived fermentation broth. The results showed that the addition of a mixture of Gastrodin elata Bl. and Tartary buckwheat alcoholic extracts compound solution could enhance the activities of PGM and UGPase while reducing GPI activity. Compared with the blank group, the activities of PGM and UGPase were increased by 86.98% and 69.12%, respectively, and the activity of GPI was decreased by 65.78%. In addition, the fermentation broth containing Gastrodin elata Bl-Tartary buckwheat alcoholic extract mixture had the greatest anti-fatigue effect, which was significantly higher than that of the blank group (p<0.05) and experimental group (p<0.05). The anti-fatigue effect, was evidenced mainly by the increases in the swimming exhaustion time, hepatic glycogen (HG) content and lactate dehydrogenase (LDH) content, and the decreases in the contents of lactic acid (LA) and blood urea nitrogen (BUN) in mice. The research shows that the addition of the extract mixture could significantly change the activities of key enzymes in the EPS synthesis pathway of Grifola frondose, thereby increasing the content of EPS in the fermentation broth and endows the both with a good anti-fatigue effect.

国家自然科学基金地区科学基金项目（31460537）；贵州省肉牛现代农业产业技术体系（GZCYTX2020-0302）；贵州省科技计划项目（黔科合支撑[2020]4Y116号）