评价本实验室研制的小肠结肠炎耶尔森氏菌微量生化鉴定试剂盒（简称EasyID）的使用性能。用36株菌（包括小肠结肠炎耶尔森氏菌2株标准株，19株分离株；弗氏耶尔森氏菌5株分离株；中间型耶尔森氏菌5株分离株；克氏耶尔森氏菌5株分离株），测试EasyID，传统液态生化管（简称HKM）以及国内其他品牌（简称KIT）的三种鉴定试剂盒。结果表明：36株测试菌株的鉴定结果，EasyID、KIT两种干制鉴定试剂盒与HKM传统液态鉴定试剂盒鉴定结果相比总体符合率分别为99.69%、98.15%，EasyID相比KIT，与HKM的符合率更高；EasyID、HKM、KIT三种鉴定试剂盒与标准要求相比总体符合率分别为99.37%、99.07%、97.83%，EasyID符合率最高，HKM其次，KIT略低。结论：EasyID小肠结肠炎耶尔森氏菌微量生化鉴定试剂盒的可靠性优于KIT，与HKM相当。在使用方便性上，采用一步加样的技术的EasyID优于HKM和KIT。

This study aimed to evaluate the performance of the micro-biochemical identification kit for Yersinia enterocolitica developed in our laboratory (termed EasyID). Thirty six strains of Y. enterocolitica (including two standard strains and nineteen isolated strains of Y. enterocolitica; five isolated strains of Y. flexneri; five isolated strains of Y. intermedia; five isolated strains of Yersinia Klebsiella) were used to evaluate the three kinds of Y. enterocolitica identification reagent kits: EasyID, traditional liquid biochemical tube (HKM) and other domestic brands (termed KIT). The results showed that the identification results of the thirty six tested strains exhibited overall coincidence rates of 99.69% and 98.15%, respectively, for the “dry” identification kits, EasyID and KIT, compared with those of the traditional liquid identification kits. Compared with KIT, EasyID had a higher coincidence rate with HKM; the three identification kits, EasyID, HKM and KIT, had overall compliance rates of 99.37%, 99.07%, and 97.83%, respectively, compared with the standard requirements, with the EasyID having the highest conformity rate, followed by HKM then KIT. Conclusion: The reliability of EasyID Yersinia enterocolitica microbiochemical identification kit was superior to KIT and comparable to HKM. In terms of ease of use, EasyID involving one-step sampling technique was superior to HKM and KIT.

国家重点研发计划项目（2018YFC1604201）；肇庆市引进西江创新团队项目（肇人才领2018-8）