GC-MS分析紫苏叶与陈皮复配前后挥发油的变化

GC-MS Analysis of the Changes in Volatile Oils before and after Compounding of Perilla Leaf and Tangerine Peel

摘要:

为探究紫苏叶、陈皮复配前后挥发油的变化，采用水蒸气蒸馏法提取紫苏叶、陈皮及紫苏叶和陈皮复配物（简称复配物）的挥发油，利用GC-MS技术对各挥发油进行分析，比较其成分及含量的差异。从紫苏叶、陈皮及复配物的挥发油中分别鉴定出20、22和31个化合物，检出物总质量分数分别为97.77%、99.76%和97.94%。紫苏叶挥发油中薄荷醇、β-波旁烯、反式-橙花叔醇和环氧化蛇麻烯II共4个化合物未在复配物挥发油中检测到，且其中β-古巴烯的含量在复配物中明显增加；陈皮挥发油中2-蒈烯、对-伞花烯、异松油烯、4-萜烯醇和大根香叶烯共5个化合物未在复配物挥发油中检测到，且其中香附烯酮的含量在复配物中明显增加；而复配物挥发油中新增加了4-蒈烯、邻-异丙基苯、癸醛、γ-榄香烯、乙酸橙花酯和乙酸橙花叔醇酯共6个化合物。因此，该研究说明紫苏叶、陈皮复配前后挥发油成分有明显变化，为紫苏叶和陈皮的进一步开发提供新思路。

Abstract:

Steam distillation was performed to extract the volatile oils of Perilla leaf, tangerine peel, and the compound of Perilla leaf and tangerine peel (hereinafter referred to as the compound) to investigate changes in volatile oils before and after the compounding of Perilla leaf and tangerine peel. The volatile oils were analyzed by gas chromatography-mass spectrometry to compare the differences in their composition and content. A total of 20, 22, and 31 components were identified from the volatile oils of Perilla leaf, tangerine peel, and the compound, respectively, and the total mass fractions of the detected substances were 97.77%, 99.76%, and 97.94%, respectively. Four components in the volatile oils of Perilla leaf (menthol, β-bourbonene, trans-nerolidol, and humulene epoxide II) were not detected in the volatile oils of the compound, whereas the content of β-copaene increased significantly after compounding. Five components in the volatile oils of tangerine peel (2-carene, p-cymene, isoterpinolene, 4-terpenol, and germacrene) were not detected in the volatile oils of the compound, and the content of cyperenone increased significantly after compounding. Six additional components were detected in the volatile oils of the compound (4-carene, o-isopropyl benzene, decanal, γ-elemene, neryl acetate, and nerolidyl acetate). The results showed that there were significant changes in the components of volatile oils before and after compounding of Perilla leaves and tangerine peel. This study provides new insights for the further development of Perilla leaf and tangerine peel.