梁王茶水提物急性毒性评价

Assessment of Acute Toxicity of Nothopanax delavayi (Franch.) Harms ex Diels Water Extract

摘要:

以掌叶梁王茶叶为原料，研究其水提物的急性毒性及半数致死量LD50。结果表明：梁王茶水提物经口LD50>15000 mg/kg•bw，属于实际无毒物质。与对照组相比，灌胃2521~15000 mg/kg•bw梁王茶水提物对雌性和雄性小鼠的体重增量无显著影响（p>0.05），对雌鼠心、脾和肺脏器系数、血清ALT、AST和肌酐水平均无显著影响（p>0.05），对雄鼠肺和肾脏器系数、血清ALT水平均无显著影响（p>0.05）。极高剂量梁王茶水提物使雌性（剂量≥7350 mg/kg•bw）和雄性（剂量≥5145 mg/kg•bw）小鼠的血清尿素水平显著高于空白对照组（p<0.05），说明极高剂量梁王茶水提物对肾脏有一定毒性。急性毒性研究表明，梁王茶水提物经口LD50>15000 mg/kg•bw，属于实际无毒物质，极高剂量时其对肾脏有一定毒性。

Abstract:

Leaves of Nothopanaxdelavayi (Franch.) Harms ex Diels (LWC), was used as raw material, the acute toxicity and half lethal dose (LD50) of its water extract was investigated. Results showed that the oral LD50 of LWC water extract was higher than 15000 mg/kg.bw, which is actually non-toxic substance. Comparing to control group, gavage of 2521~15000 mg/kg•bw LWC water extract had no significant effect on the weight gain of both female and male mice (p>0.05), showing insignificant influence on organ coefficients of heart, spleen and lung, serum ALT, AST and creatinine level of female mice (p>0.05), neither were lung and kidney organ coefficients, serum ALT of male mice (p>0.05). The serum urea level of female (dose ≥7350 mg/kg•bw) and male (dose ≥5145 mg/kg•bw) mice was significantly higher than that of the blank control group (p<0.05), indicating that the very high dose of LWC tea water extract had a certain toxicity to the kidney. In summary, acute toxicity studies have shown that the oral LD50 of LWC water extract is more than 15000 mg/kg•bw, which is a practically non-toxic substance, however it is toxic to the kidneys at extremely high doses.