Abstract:To study and analyze the growth and toxin production law of Pseudomonas cocovenenans subsp farinofermantans (hereinafter referred to as “Pseudomonas cocovenenans”) and the associated risks in three food matrixes, different concentration(103, 105 and 107 cfu/mL) Pseudomonas cocovenenans was added into wet rice noodle, its raw rice and rice pulp, then these foods were cultured at the best toxin production temperature (26 ℃) and the best growth temperature (36 ℃) and toxin (i.e., bongkrek acid) content changes among them were determined. The result shows that bongkrek acid production of Pseudomonas cocovenenans was positively correlated with the quantity of Pseudomonas cocovenenans in food matrix and had a great correlation with food properties and water content. Bongkrek acid production in wet rice noodle is much higher than that in its raw rice and rice pulp under the situation of same quantity of strain, cultured temperature and time (Up to about 5000 times). When the inoculation concentration in wet rice noodle was 105 cfu/mL and cultured at 26℃ and 36℃ for 48 hours, bongkrek acid content could reach 27 mg/kg, it was high risk level, bongkrek acid content in raw rice during 35 days cultured was in the range of 0.7~16 µg/kg, it was low risk level. The study suggests that the whole chain of raw materials, production, storage and transportation, management and consumption should be strengthened to prevent and control the pollution of wet rice noodle by Pseudomonas cocovenenans.
