蔓越莓制品对斑马鱼抗感染及炎症的抑制作用

Anti-infection and Anti-inflammatory Effects of Cranberry Products on Zebrafish

摘要:

为研究蔓越莓果汁粉（MYMFA）、蔓越莓全果粉（MYMFB）、蔓越莓提取物粉（MYMFC）与市售的高浓度蔓越莓精华（High Strength Cranberry）等四种蔓越莓制品的抑菌与抗炎作用，该研究以三金片作为阳性对照，首先确定了三金片与4种蔓越莓制品对斑马鱼的无可见有害作用水平（No Observed Adverse Effect Level，NOAEL）分别为1000 μg/mL和2000 μg/mL。以此为根据，分别选取低、中、高三个浓度对被感染P菌毛阳性大肠杆菌的转基因中性粒细胞荧光斑马鱼进行饲养试验，根据肠腔中细菌的平均荧光强度和中性粒细胞数量评价上述蔓越莓制品抑菌和抗炎能力。结果表明：三金片与四种待测品在高浓度时对斑马鱼的细菌感染和炎症反应均有极显著的抑制作用（***p<0.001）。其中MYMFC的抑制效果最好，在高浓度下其抑菌率达到60.38%，炎症消除率达到51.16%，均超过三金片的抑菌率（53.84%）和炎症消除率（46.51%）。以上结果表明，蔓越莓制品具有较好的抑菌抗炎效果，且MYMFC在保证安全性的同时显示出比三金片更加优越的抑菌抗炎能力。

Abstract:

The antibacterial and anti-inflammatory effects of four types of cranberry products, including cranberry juice powder, cranberry whole fruit powder, cranberry extract powder (MYMFC), and commercially available concentrated cranberry extract (high-strength cranberry) was evaluate. Sanjin tablets, a commercially available Chinese medicine, was used as a positive control. First, the No Observed Adverse Effect Levels (NOAEL) of the Sanjin tablets and four cranberry products on zebrafish were determined as 1000 and 2000 μg/mL, respectively. Next, low, medium, and high concentrations of these products were used for feeding experiments using transgenic zebrafish with neutrophil fluorescence infected with P fimbriae-positive Escherichia. coli. The antibacterial and anti-inflammatory effects of the cranberry products were determined by measuring the average fluorescence intensity of bacteria in the intestinal lumen and number of neutrophils. The results showed that Sanjin tablets and the four tested cranberry products at high concentrations had extremely significant inhibitory effects (***p < 0.001) on bacterial infection and inflammation in zebrafish, with MYMFC showing the strongest effect. At high concentrations, its bacterial inhibition rate reaches 60.38%, and the inflammation inhibition rate reached 51.16%; these values exceed those of Sanjin tablets (53.84% and 46.51%, respectively). These results demonstrate that cranberry products are safe and have strong antibacterial and anti-inflammatory effects, with MYMFC showing superior antibacterial and anti-inflammatory capabilities compared to those of Sanjin tablets.