该实验研究了芥菜疙瘩超微粉（BUP）对脂多糖鼻腔滴注构建的慢性阻塞性肺疾病（COPD）小鼠肺炎的改善作用。结果显示，与粉碎后过60目筛的芥菜疙瘩普通粉（BOP）相比，BUP的活性成分可溶性膳食纤维、多酚、黄酮、硫苷、异硫氰酸酯等具有更高的溶出率（分别提高了77.27%、63.89%、17.86%、26.72%、42.86%）；BUP可以改善COPD小鼠的体重减轻、肺气肿和肺纤维化，提高COPD小鼠肺泡灌洗液（BALF）中调节性T细胞的比例，降低巨噬细胞、树突状细胞和中性粒细胞的比例，下调COPD小鼠肺组中IL-1β和IL-6的水平并上调IL-10的水平，抑制肺组织内IκB和NF-κB p65的磷酸化，阻止p65入核促进炎症因子的表达；同时BUP能够平衡COPD小鼠BALF中活性氧水平，改善其肺组织内一氧化氮（NO）、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）等氧化应激相关因子的水平，促进肺组织内Nrf2、血红素加氧酶-1（HO-1）和醌氧化还原酶-1（NQO-1）的表达。研究表明BUP可能由于其活性成分的溶出率优于BOP从而具有更好的改善COPD小鼠肺炎的作用，其机制与抗炎和抗氧化作用相关。实验结果可为芥菜疙瘩的开发利用提供新的参考依据。

The ameliorating effect of Brassica napiformis superfine powder (BUP) on the mice with chronic obstructive pulmonary disease (COPD) induced by intranasal instillation of lipopolysaccharide was investigated. The results showed that the active ingredients of BUP, such as soluble dietary fiber, polyphenols, flavonoids, glucosinolates and isothiocyanates, had higher dissolution rates (which are increased by 77.27%, 63.89%, 17.86%, 26.72% and 42.86% respectively) compared with the Brassica napiformis powder (BOP) that was prepared via crushing and passing through a 60-mesh sieve; BUP could ameliorate weight loss, emphysema and pulmonary fibrosis in COPD mice, increase the proportion of regulatory T cells in broncho alveolar lavage fluid (BALF) of COPD mice, reduced the proportion of macrophages, dendritic cells and neutrophils, and down-regulate the levels of IL-1β and IL-6 while up-regulating IL-10 level in the lung tissues of COPD mice. BUP could prevent from p65 entering the nucleus to promote the expression of inflammatory factors through inhibiting the phosphorylation of IκB and NF-κB p65 in lung tissues. At the same time, BUP could balance the level of reactive oxygen species in the BALF of COPD mice, improve the levels of oxidative stress-related factors, such as nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and promote the expressions of Nrf2, heme oxygenase-1 (HO-1) and quinone oxidoreductase-1 (NQO-1) in the lung tissues of COPD mice. The research results showed that the ameliorating effect of BUP on COPD mice was greater than that of BOP, due to the higher dissolution rate of BUP’s active ingredients, and the underlying mechanism might be related to its anti-inflammatory and antioxidant effects. The experimental results provide a new reference for the development and utilization of Brassica napiformis.

临泉县-合肥工业大学产业创新引导资金项目（113-413357）