Abstract:In order to establish a new green and efficient process for extracting anthocyanins from mulberry, mulberry was used as the raw material and deep eutectic solvents (DESs) was used as the extractant to explore the effects of the types of DESs, water content, solid-liquid ratio, as well as extraction temperature and extraction time on the extraction efficiency of anthocyanins. On the basis of single factor experiments, the extraction conditions were optimized by response surface methodology. The results revealed that the yield of anthocyanins was 6.35 mg/g when the choline chloride/acetic acid (1/3 n/n) -water (80/20 V/V) was selected as the suitable solvent, solid-liquid ratio was 1/10 (g/mL), extraction temperature was 60 ℃ and extraction time was40 min. The experimental result was close to the predicted value of the response surface model, indicating that the model is suitable. The extraction efficiency of this process for mulberry anthocyanins was much higher than that of traditional extractants, suggesting the strong hydrogen bonding between DESs and the target product (which was composed of a proper ratio of hydrogen bond donors and acceptors) was more conducive to the leaching and dissolution of anthocyanins. The results of this study provide a reference for high-efficiency preparation of natural products with green and designable DES solvents.
