桔甘片对急性酒精性肝损伤小鼠的保护作用

Protective Effect of Jie-Gan Tablets on Alcoholic-induced Acute Liver Injury in Mice

摘要:

探讨桔甘片（Jie-Gan Tablets，JGT）对急性酒精肝损伤小鼠的保护作用。采取50%酒精一次性灌胃（i.g）的方法建立小鼠急性酒精性肝损伤模型。检测小鼠血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）、甘油三酯（TG）水平，肝组织匀浆中丙二醛（MDA）的含量及谷胱甘肽过氧化酶（GSH-Px）的活性，采用H&E染色分析组织切片形态变化，评估JGT的肝保护作用。结果显示不同剂量JGT和联苯双酯预处理能够显著抑制小鼠体内两种血清转氨酶水平的升高（p<0.05），抑制率最高达到61.81%。JGT高剂量组显著降低血清中TG水平（p<0.05），降幅达到34.73%。与模型组相比，阳性对照组显著逆转了MDA水平上升这一现象（p<0.01），JGT各给药组呈剂量依赖的方式降低MDA的含量（p<0.05），且提高了抗氧化酶GSH-Px的活性（p<0.05），其中高剂量组MDA含量降至1.74 nmol/mg，GSH-Px活力上升至828.81 U/mg pro。H&E染色结果表明，JGT预处理能够有效改善酒精诱导的肝损伤。由此可见，JGT对ALD具有一定的预防保护作用。

Abstract:

The protective effect of Jie-Gan tablets (JGT) on acute alcohol liver injury mice was explored. A mouse model of acute alcoholic liver injury was established by one-time intragastric gavage (i.g) with 50% alcohol. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and triglyceride (TG), and the content of malondialdehyde (MDA) and activity of glutathione peroxidase (GSH-Px) in the liver tissue homogenate of mice were determined. The morphological changes of tissue sections were analyzed by H&E staining, and the hepatoprotective effect of JGT was evaluated. The results showed that the increases of the two serum transaminase levels in mice were significantly inhibited by the pretreatments with different doses of JGT and bifendatatum (p<0.05), and the highest inhibition rate up to 61.81%. The serum TG level was significantly reduced in the JGT high-dose group (p<0.05), with a decrease of 34.73%. Compared with the model group, the increased MDA level was significantly reversed in the positive control group (p<0.01). The content of MDA was reduced in a dose-dependent manner in each of the JGT administration group (p<0.05), and their antioxidant enzyme GSH-Px activities increased (p<0.05). Among which, the MDA content in the high-dose group was reduced to 1.74 nmol/mg, and the GSH-Px activity increased to 828.81 U/mg pro. H&E staining results showed that alcohol-induced liver injury was effectively alleviated by JGT pretreatment. Accordingly, JGT has a certain preventive and protective effect on ALD.