甲基烯丙基三硫醚对溃疡性结肠炎小鼠炎症因子及肠道菌群的调节作用

Regulating Effects of Allyl Methyl Trisulfide on Inflammatory Factor and Intestinal Flora in Ulcerative Colitis Mice

摘要:

该研究探究了甲基烯丙基三硫醚（allyl methyl trisulfide，AMTS）对溃疡性结肠炎小鼠炎症因子及肠道菌群的调节作用。观察小鼠体重、结肠长度、疾病活动指数（DAI）及结肠组织变化，ELISA检测血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）水平，16S rRNA系统测序技术检测肠道菌群的变化。研究表明模型组体重下降25.59%，AMTS低、中、高剂量组分别下降4.06%、9.88%和5.45%。模型组DAI值为3.51，AMTS三个剂量组分别为2.60、2.88和2.79。与模型组相比，AMTS的三个干预组都减轻了结肠组织炎症细胞浸润，同时都显著性降低了结肠中促炎细胞因子TNF-α、IL-1β、IL-6的水平（p<0.01，p<0.05）。AMTS组较模型组可增加肠道菌群丰度及多样性。与模型组相比，AMTS还能降低拟杆菌、变形菌等数量，提高厚壁菌、乳酸杆菌等有益菌群的数量。研究表明AMTS可通过降低炎症因子水平和增加肠道菌群的多样性缓解溃疡性结肠炎小鼠的症状。

Abstract:

The regulating effects of allyl methyl trisulfide (AMTS) on inflammatory factor and intestinal flora in ulcerative colitis mice were investigated in this study. Body weight, colon length, disease activity index (DAI) and histological changes were observed. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) contents in serum were detected by ELISA method. And the intestinal flora diversity in mice feces was analyzed by 16s rDNA high throughput sequencing. The results showed that the weight of the model group decreased by 25.59%, and the low-dose, medium-dose and high-dose AMTS groups were decreased by 4.06%, 9.88% and 5.45% respectively. The DAI in model group was 3.51, the three AMTS groups were 2.60, 2.88 and 2.79, respectively. Compared with the model group, three dosages of AMTS groups ameliorated inflammatory infiltration in colonic tissue, and significantly decreased the levels of pro-inflammatory cytokines, like TNF-α, IL-1β, IL-6 (p<0.01, p<0.05). The abundance and diversity of intestinal flora were increased after administration with AMTS. Compared to the model group, the microbial abundance of Bacteroidetes, Proteobacteria were decreased, and the abundance of Firmicutes, Lactobacillus were increased in the AMTS groups. In conclusion, the results indicate that AMTS alleviates the symptoms of ulcerative colitis by reducing the level of inflammatory factors and increasing the abundance and diversity of intestinal flora in mice.