该研究探讨元宝枫籽油对脂多糖（LPS，3.5 mg/kg）诱导雄性ICR小鼠肠道炎症的改善作用机制。连续7 d灌服元宝枫籽油后观察小鼠体重、结肠的变化，玉米油为对照。酶联免疫法测定血清及结肠中白介素（interleukin，IL）-1β、IL-6、IL-18和肿瘤坏死因子-α（TNF-α）水平。qRT-PCR法检测结肠内NOD样受体蛋白3（Nlrp3），凋亡相关斑点样蛋白（Asc），半胱氨酸蛋白酶-1（Caspase-1）和Il1β的mRNA水平。元宝枫籽油显著抑制LPS致小鼠体重下降，降低小鼠结肠重量与长度比（p<0.05）。元宝枫籽同时抑制LPS致损伤小鼠血清炎性因子（L-1β：155.66 ng/L、IL-6：75.42 ng/L、IL-18：90.31 ng/L和TNF-α：47.97 ng/L）及结肠炎性因子（IL-1β：188.85 ng/L、IL-6：57.88 ng/L、IL-18：52.29 ng/L和TNF-α：85.69 ng/L）水平；显著降低小鼠结肠髓过氧化物酶水平至81.53 ng/L（vs损伤组：113.59 ng/L，p<0.05），丙二醛水平至1.04 μmol/g protein（vs损伤组：1.60 μmol/g protein，p<0.05）。此外，与损伤组相比，元宝枫籽油分别抑制Nlrp3（41.80%）、Asc（49.85%）、Caspase-1（31.28%）和Il1β（39.83%）的mRNA表达。综上所述，元宝枫籽油能改善LPS致小鼠肠道炎症反应的机制可能与其抑制结肠内NLRP3小体过度活化有关。

The effect and mechanism of Acer truncatum Bunge seed oil on lipopolysaccharide (LPS, 3.5 mg/kg) intraperitoneal injection induced intestinal inflammation in male ICR mice were investigated. After 7 days, Acer truncatum Bunge seed oil was administrated by gavage, and corn oil was used as the control. The body weight and colon tissue of mice were observed. The levels of cytokines, including IL-1β, IL-6, IL-18 and TNF-α in serum and colon were measured by ELISA assay, respectively. The mRNA levels of Nlrp3, Asc, Caspase1 and Il1β in colon were detected by qRT-PCR assay. Acer truncatum Bunge seed oil can significantly inhibit the weight loss and reduce the ratio of colon weight to length in LPS-treated mice (p<0.05). At the same time, the levels of IL-1β (155.66 ng/L and 188.85 ng/L), IL-6 (75.42 ng/L and 57.88 ng/L), IL-18 (90.31 ng/L and 52.29 ng/L) and TNF-α (47.97 ng/L and 85.69 ng/L) in serum and colon of the model mice were significantly reduced by the intervention of Acer truncatum Bunge seed oil. The levels of MPO and MDA in colon of model mice were significantly reduced to 81.53 ng/L (vs injury group: 113.59 ng/L, p<0.05) and to 1.04 µmol/g protein (vs injury group: 1.60 µmol/g protein, p<0.05). Compared with the injured group, Acer truncatum Bunge seed oil inhibited the mRNA expression of Nlrp3 (41.80%), Asc (49.85%), Caspase1 (31.28%) and Il1β (39.83%). These results suggest that Acer truncatum Bunge seed oil improve the intestinal inflammation in LPS-treated mice. The mechanism may be associated with the reducing the activation of NLRP3 by Acer truncatum Bunge seed oil in LPS-treated mice.

国家自然科学基金项目（81560530；81760589；81960590）；人社部“高层次留学人才回国资助计划”（人社厅函[2019]160号）；广西自然科学基金项目(2020GXNSFAA159160)；广西高等学校千名中青年骨干教师培育计划资助项目（桂教人[2018]18号）；桂林医学院引进人才科研启动基金项目（04010150001）