大孔吸附树脂纯化吴茱萸果实多糖工艺优化

Optimization of the Purification Process for the Polysaccharides from Tetradium ruticarpum Fruit Using Macroporous Adsorption Resins

摘要:

为得到大孔吸附树脂对吴茱萸果实多糖纯化的最佳工艺，本研究利用水提醇沉法提取多糖，考察7种不同类型大孔吸附树脂对该多糖的纯化效果，并利用静态和动态单因素吸附实验及响应面法优化树脂纯化工艺，最后对吸附前后多糖部分进行表征。结果表明，S-8树脂对吴茱萸果实多糖具有较好的综合纯化效率；最佳工艺参数如下：样品浓度为4.41 mg/mL，初始pH值为5，吸附环境温度24.30 ℃，流速2.0 BV/h；该条件下色素清除率为49.21%，蛋白清除率为68.97%，多糖保留率为55.05%。结构表征结果表明，吸附前后该多糖分子量（Mw）分布（主峰1 Mw>2.00×105 u，主峰2 Mw约为2.35×104 u），红外吸收特征及糖链异头氢吸收信号（δ 5.04×10-6和δ 4.42×10-6）等均未发生明显改变。综上，S-8大孔吸附树脂可用于吴茱萸果实多糖的高效纯化，条件温和环保，且不会引起多糖链断裂和多糖结构改变。

Abstract:

In order to obtain the best purification process using macroporous resin for the polysaccharides from Tetradium ruticarpum fruit (TrPs), water extraction and alcohol precipitation were applied to extract the polysaccharides. The purification performance of seven different types of macroporous adsorption resins was examined by used static and dynamic single factor experiments, and the purification process was optimized by the response surface method. Finally, the polysaccharide structure before and after adsorption was characterized. The results show that the S-8 resin had the highest comprehensive purification efficiency for the crude TrPs, and the optimal purification conditions were: sample concentration, 4.41 mg/mL; initial pH 5; adsorption environment temperature, 24.30 ℃; flow rate 2.0 BV/h. Under these conditions, the decolorization rate was 49.21%, deproteinization rate was 68.97% and polysaccharide retention rate was 55.05%. The structural characterization results revealed the molecular weight (Mw) distribution of the polysaccharide before and after adsorption (Mw of peak 1 higher than 2.00×105 u; Mw of peak 2, approximately 2.35×104 u), the infrared absorption characteristics and the sugar chain anomeric hydrogen absorption signal of TrPs (δ 5.04×10-6 and δ 4.42×10-6) did not change significantly before and after adsorption. In summary, S-8 macroporous adsorption resin can be used for the high-efficiency purification of TrPs, under mild and environmentally friendly conditions, without causing polysaccharide chain scission and polysaccharide structural changes.