芥子酸改善高脂饮食模式下叙利亚仓鼠脂代谢与氧化应激水平

Sinapic Acid Ameliorated Lipid Metabolism and Oxidative Stress in High Fat Diet Fed Syrian Hamsters

摘要:

探讨芥子酸对高脂饮食仓鼠脂代谢和氧化应激的影响。比色法分别检测仓鼠血清和肝脏中总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-c）、低密度脂蛋白（LDL-c）、游离脂肪酸（NEFA），丙二醛（MDA）和总抗氧化力（T-AOC）水平。蛋白印迹法检测肝脏中PPAR-γ、CPT-1、ACC1、FAS、HMGCR、SREBP2和CYP7A1的表达。与高脂饮食组相比，芥子酸干预显著降低肥胖仓鼠体重（23.86%）、附睾脂肪（20.38%）和肾周脂肪（27.91%）指数（p<0.05）；改善血脂水平（至TG：183.51 μmol/L、TC：1481.67 μmol/L、HDL-c：507.81 μmol/L、LDL-c：272.50 μmol/L和NEFA：1.16 ng/L）；分别降低MDA水平（至血清：7.85 nmol/L，肝脏：5.47 nmol/L）并提升T-AOC水平（至血清：12.63 U/mL，肝脏：113.72 U/mL）。芥子酸干预还能较高脂饮食组仓鼠显著提高PPAR-γ（2.00倍）、CPT-1（2.61倍）、CYP7A1（1.65倍），并显著抑制FAS（34.38%）、ACC1（52.73%）、SREBP2（53.49%）和HMGCR（32.68%）的表达水平（p<0.05）。本研究提示，芥子酸能够显著改善高脂膳食诱导的仓鼠脂代谢紊乱，这与调控脂代谢相关蛋白的表达和减轻氧化应激水平有关。

Abstract:

To investigate the effect of sinapic acid on lipid metabolism and oxidative stress in a high-fat diet (HFD) fed Syrian hamsters, the serum and hepatic levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL-c), low densitylipoprotein (LDL-c), nonesterified free fatty acids (NEFA) and malondialdehyde (MDA) and total antioxidant capacity (T-AOC) were determined by colorimetric method. The hepatic levels of lipid metabolism related proteins, which including peroxisome proliferator-activated receptor gamma (PPAR-γ) carnitine palmitoyltransferase-1 (CPT-1), acetyl-CoA carboxylase1 (ACC1), fatty acid synthase (FAS), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), sterol regulatory element binding protein-2 (SREBP2) and cytochrome P450 family 7 subfamily A member 1 (CYP7A1) were determined using a Western Blotassay. Administrated with sinapic acid can significantly reduce the weight of HFD hamsters (23.86%), epididymal fat (20.38%) and perirenal fat (27.91%) (p<0.05) compared with HFD fed hamsters. Sinapic acid treatment also attenuated the serum lipid levels (TG: 183.51 μmol/L, TC: 1481.67 μmol/L, HDL-c: 507.81 μmol/L, LDL-c: 272.50 μmol/L and NEFA: 1.16 ng/L). The MDA levels were reduced to 7.85 nmol/L and 5.47 nmol/L, and T-AOC levels were increased to 12.63 U/mL and 113.72 U/mL in serum and liver of the model hamsters, respectively, by the intervention of sinapic acid. In addition, sinapic acid can promote the protein expression of PPAR-γ (2.00 times), CPT-1 (2.61 times), CYP7A1 (1.65 times) and inhibit the protein expression of FAS (34.38%), ACC1 (52.73%), SREBP2 (53.49%) and HMGCR (32.68%) compared with HFD hamsters. Our results suggested that sinapic acid can significantly improve lipid metabolism disorders induced by HFD in hamsters, which is related to the regulation of lipid metabolism-related proteins and the reduction of oxidative stress levels.