本研究建立了荔枝皮中儿茶素（C）、表儿茶素（EC）、没食子酸（GA）、原花青素B2（PCB2）、原花青素B4（PCB4）和原花青素A2（PCA2）含量的测定方法。样品经85%乙醇水溶液提取，Oasis PRiME HLB固相萃取柱净化，高效液相色谱（HPLC）同时进行检测。色谱柱为Thermo Syncronis C18（250×4.6 mm，5 μm），以甲醇和1%冰乙酸溶液为流动相，梯度洗脱，流速1.0 mL/min，柱温35 ℃，检测波长280 nm。儿茶素、表儿茶素、没食子酸、原花青素B2、原花青素B4和原花青素A2在0.5~100 mg/L范围内与峰面积呈良好的线性关系，相关系数均大于0.9998。平均添加回收率为83.00%~102.00%，相对标准偏差（RSD）在0.48%~0.83%之间。儿茶素、表儿茶素、没食子酸、原花青素B2、原花青素B4和原花青素A2在荔枝皮中检出限分别为0.52，0.55，0.35，0.45，0.95和0.65 mg/kg，定量限分别为1.26、1.64、0.95、1.35、2.80和1.45 mg/kg。该方法简便、快速、准确，重复性好，可用于荔枝皮中C、EC、GA、PCB2、PCB4、PCA2的含量同时定量测定。

A method was established in this study for determining the contents of catechin (C)，epicatechin (EC)，gallic acid (GA)，procyanidin B2 (PCB2)，procyanidin B4 (PCB4) and procyanidin A2 (PCA2) in litchi pericarp. The samples were extracted with 85% aqueous ethanolic solution and purified by Oasis PRiME HLB solid-phase extraction column, and analyzed by HPLC. The chromatographic column was thermo Syncronis C18 (250×4.6 mm, 5 μm), with methanol and 1 % glacial acetic acid as the mobile phase in gradient elution at a flow rate of 1.0 mL/min, column temperature at 35 ℃ and detection wavelength at 280 nm. The separation was carried out using consisting of. In the range of 0.5~100 mg/L, the concentrations of catechin, epicatechin, gallic acid, procyanidin B2, procyanidin B4 and procyanidin A2 had a good linear relationship with the peak areas with coefficients all higher than 0.9998. The average spiking recovery rates were 83.00%~102.00%, with relative standard deviations (RSD) between 0.48% and 0.83 %. The limits of detection (LOD, S/N=3) were 0.52, 0.55, 0.35, 0.45, 0.95 and 0.65 mg/kg, and the limits of quantification (LOQ, S/N=10) were 1.26, 1.64, 0.95, 1.35, 2.80 and 1.45 mg/kg for catechin, epicatechin, gallic acid, procyanidin B2, procyanidin B4 and procyanidin A2, respectively. This method is simple, rapid, accurate, reproducible, and can be used for the simultaneous quantitative determination of catechin, epicatechin, gallic acid, procyanidin B2, procyanidin B4 and procyanidin A2 in litchi pericarp.

广西自然科学基金面上项目(2020GXNSFAA159073)；广西科技重大专项(桂科AAXXX)（桂科AA17204043-2)；科技先锋队‘强农富民’‘六个一’专项行动(桂农科JZ202020)；广西农业科学院基本科研业务专项资助项目(桂农科2021YT139、桂农科2021YT138)