Abstract:The laxative effect and the regulation of intestinal flora of complex sugar substitutes were investigated by mice experiment and in vitro bacterial proliferation experiment in this study. To evaluate the laxative effect of compound substitute sugar, Balb/C mice were divided into blank control group, model group, positive control group, compound glucose-substituted G1 group (0.75 g/kg·BW), G2 group (1.5 g/kg·BW), G3 group (2.25 g/kg·BW), G4 group (3 g/kg·BW) and G5 group (3.75 g/kg·BW) to establish constipation model with loperamide, and phenolphthalein and five different concentrations of complex substitute sugars were used for intervention. The weight of mice before and after the experiment, the advance rate of ink, the time of the first black defecation, the number of defecation grains within 6 h and the weight of defecation were observed. The effect of compound sugars on the proliferation of intestinal flora in mice was explored through in vitro culture experiments. The results showed that five different doses of compound sugar substitute intervention group could promote the defecation of mice, among which the defecation effect of 3.75 g/kg·BW group was the most significant. Compared with the model group, the ink propulsion rate increased by 48.47%, the first black stool time decreased by 67.25 min, the number of defecation particles increased by 8.75 n in 6 h, and the fecal weight increased by 0.20 g in 6 h. The in vitro culture experiment showed that compound sugar at the concentration of 0.75 g/mL, 0.15 g/mL, 0.225 g/mL, 0.3 g/mL and 0.375 g/mL could promote the proliferation of Bifidobacterium and Lactobacillus and inhibit the proliferation of Enterobacter and Enterococcus. In the concentration from 0.075 g/mL to 0.375 g/mL, the higher the concentration of compound sugar, the more obvious the trend of promoting the proliferation of Bifidobacterium and Lactobacillus. Therefore, the compound sugar substitute in this study has a laxative effect and can regulate the intestinal flora.
