牛乳酪蛋白源ACE抑制肽对大鼠的降血压作用

Antihypertensive Effect of the ACE Inhibitory Peptides Derived from Bovine Casein in Rats

摘要:

为了考察牛乳酪蛋白源血管紧张素转化酶抑制肽（Angiotensin I-converting enzyme inhibitory peptides，ACEI）的降血压作用，以自发性高血压大鼠（Spontaneously hypertensive rats，SHR）为动物模型，给予样品30 d后，检测大鼠的血压、心率、血清及尿液。与模型对照组相比，ACE抑制肽高、低剂量组和阳性对照组的收缩压分别为175、175、172 mm Hg，差异具有统计学意义（p<0.01）；ACE抑制肽高剂量组的降钙素基因相关肽（Calcitonin gene related peptide，CGRP）浓度升至10.09 pg/mL，差异具有统计学意义（p<0.05）；ACE抑制肽高剂量组的总钙（TCa）、内皮素-1（Endothelin-1，ET1）、血糖（Glucose，GLU）、总胆固醇（Cholesterol，CHOL）、高密度脂蛋白胆固醇（High density liptein cholesterol，HDL-C）、血尿酸（Uric acid，UA）浓度明显降低，分别降至2.06 mmol/L、8.69 pg/mL、6.41 mmol/L、1.13 mmol/L、0.29 mmol/L、108.20 μmol/L，差异具有统计学意义（p<0.05）。结果说明，在本实验条件下，ACE抑制肽不仅具有缓解大鼠心率过速与明显的降血压作用，也有助改善肾血管性高血压大鼠血清生化指标及肾功能指标，同时对血糖、血尿酸以及胆固醇有一定控制作用。ACE抑制肽可能通过升高血液CGRP、抑制ET1、降低血钙水平起到降血压作用。

Abstract:

In order to investigate the blood pressure-lowering effect of angiotensin I-converting enzyme inhibitory peptides (ACEI) derived from bovine casein, spontaneously hypertensive rats (SHR) were used as the animal model. After 30 days of sample administration, the blood pressure, heart rate, serum and urine of rats were examined. Compared with the model control group, the systolic blood pressure and heart rate of the ACE inhibitory peptides high- and low-dose groups and the positive control group were 175, 175, and 172 mm Hg, respectively, with the differences being statistically significant (p<0.01); the concentration of calcitonin gene-related peptide (CGRP) in the ACE inhibitory peptides high-dose group increased to 10.09 pg/mL, with the change being statistically significant (p<0.05); the concentrations of total calcium (TCa), endothelin-1 (ET1), blood glucose (GLU), total cholesterol (CHOL), high density lipoprotein cholesterol (HDL-C) and blood uric acid (UA) in the ACE inhibitory peptides high-dose group decreased significantly (to 2.06 mmol/L, 8.69 pg/mL, 6.41 mmol/L, 1.13 mmol/L, 0.29 mmol/L and 108.20 μmol/L, respectively), with the changes being statistically significant (p<0.05). The results showed that under the experimental conditions, ACE inhibitory peptides not only relieved the fast heartbeat and lowered significantly blood pressure in rats, but also help to improve the serum biochemical indices and renal function indices of rats with renovascular hypertension while exhibiting a certain monitoring effect on the blood sugar, blood uric acid and cholesterol. ACE inhibitory peptides may lower blood pressure by increasing blood CGRP, inhibiting ETI, and lowering blood calcium level.