牡蛎肽与大豆肽、胶原肽、紫苏籽肽复配促进睾丸间质细胞生成雄性激素

The Combination of Oyster Peptides, Soybean Peptides, Collagen Peptides, and Perilla Seed Peptides Promotes the Production of Male Hormones in Testicular Stromal Cells

摘要:

分析牡蛎肽、大豆肽、胶原肽和紫苏籽肽的氨基酸组成，并研究牡蛎肽与大豆肽、胶原肽、紫苏籽肽以不同比例复配作用于小鼠睾丸间质细胞（TM3）分泌睾酮、二氢睾酮等雄性激素的影响，筛选适合改善性功能的低聚肽组合。采用四甲基偶氮唑蓝（MTT）比色法测定牡蛎肽与大豆肽、胶原肽、紫苏籽肽复配对TM3细胞存活率的影响，酶联免疫吸附法检测睾酮、二氢睾酮、环磷鸟苷（cGMP）的含量，硝酸盐还原酶法检测一氧化氮（NO）的含量、WST-8法检测超氧化物歧化酶（SOD）活力、硫代巴比妥酸法检测丙二醛（MDA）浓度。结果表明，四种低聚肽富含精氨酸，尤其紫苏籽肽的精氨酸含量最高为10.02%；较对照组，牡蛎肽和紫苏籽肽（浓度均为400 μg/mL，体积2:1）复配效果最好，对雄性激素睾酮、二氢睾酮、cGMP的促进作用分别是50.64%、143.69%、88.44%；此外也能提高TM3细胞NO浓度和SOD酶活，降低TM3细胞MDA浓度，维持细胞内稳定的氧化环境，表明牡蛎肽和紫苏籽肽复配促进TM3细胞生成雄性激素，具有改善男性性功能的作用。

Abstract:

The amino acid composition of oyster peptides, soy peptides, collagen peptides and perilla peptides were analyzed. Oyster peptide, soy peptide, collagen peptide, and perilla seed peptide are compounded in different proportions to affect the secretion of testosterone, dihydrotestosterone and other male hormones in mouse leydig cells (TM3) and the oligopeptide combinations were screened for improving sexual function. Tetramethylazozol blue (MTT) colorimetric method was used to determine the effect of oyster peptides, soy peptides, collagen peptides, and perilla peptides on the survival rate of TM3 cells. ELISA was used to detect testosterone, dihydrotestosterone, cyclic GMP (cGMP) content, nitrate reductase method was studied to detect nitric oxide (NO) content, WST-8 method and thiobarbituric acid method were used to detect superoxide dismutase (SOD) enzyme activity and malondialdehyde (MDA) concentration, respectively. The results showed that the four oligopeptides were rich in arginine, especially the perilla peptide, which had the highest arginine content 10.02%. Compared with the control group, oyster peptide and perilla seed peptide (both at a concentration of 400 μg/mL, volume 2:1) had the best combined effect, and the promotion effects on testosterone, dihydrotestosterone, and cGMP were 50.64%, 143.69%, and 88.44%, respectively. In addition, they can also increase the NO concentration and SOD activity of TM3 cells, and reduce the concentration of TM3 cells; maintain a stable oxidizing environment in the cells. In summary, it is shown that the combination of oyster peptide and perilla seed peptide promotes the production of male hormones by TM3 cells and has the effect of improving male sexual function.