为了探究马钱苷对细胞脂肪变性的抑制作用及相关机制，以50%胎牛血清诱导L02细胞建立脂肪变性模型，通过MTT法和油红O染色法测定了马钱苷对脂变细胞增殖的抑制情况，并采用相应试剂盒测定了实验细胞上清液中谷草转氨酶（aspartate aminotransferase，AST）、谷丙转氨酶（alanine aminotransferase，ALT）和乳酸脱氢酶（lactate dehydrogenase，LDH）酶活、总胆固醇（total cholesterol，TC）和甘油三酯（triglycerides，TG）水平以及细胞内TC、TG和硫代巴比妥酸反应物（thiobarbituric acid reactive substances, TBARS）水平。MTT结果显示，与模型组相比，马钱苷各浓度组可显著抑制脂变细胞增殖（p<0.05），抑制率为14.6%~26.91%；油红O观察发现，马钱苷处理能够剂量依赖性减少胞浆内脂滴累积；与模型组相比，马钱苷处理组细胞胞外AST、ALT和LDH酶活分别下降26.70%~52.58%、24.83%~61.23%和14.53%~28.83%，胞外TC和TG水平分别下降50.46%~68.76%和32.51%~53.37%，胞内TC和TG水平分别下降11.48%~32.79%和10.50%~18.30%，胞内TBARS水平下降40.33%~60.10%，呈现明显剂量-效应关系，且效果均强于自然恢复组。实验结果表明，马钱苷能够显著减少因脂质代谢紊乱和脂质过氧化引起的L02细胞损伤，抑制胎牛血清诱导的L02细胞脂肪变性。

In this research, 50% fetal bovine serum (FBS)-induced steatosis in human liver cell line L02 cell was used as model to investigate the therapeutic effects of loganin on cellular steatosis. The inhibitive effect of loganin on cell proliferation was detected with MTT method and Oil red O staining method. The enzymatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) and levels of total cholesterol (TC) and triglycerides (TG) in cell supernatant, as well as the intracellular levels of TC, TG and thiobarbituric acid reactive substances (TBARS) were determined with corresponding assay kits. MTT results showed that compared with the model group, different concentrations of loganin could significantly inhibit the growth of steatosis L02 cells (p<0.05), the inhibitory rate was 14.6%~26.91%. Oil red O staining showed that the accumulation of hepatic lipid in the loganin treatment groups was significantly decreased with the increase in concentration. Compared with the model group, the extracellular enzyme activities of AST, ALT and LDH in loganin treatment groups were reduced by 26.70%~52.58%, 24.83%~61.23% and 14.53%~28.83%, respectively; extracellular levels of TC and TG were reduced by 50.46%~68.76% and 32.51%~53.37%, respectively; intracellular levels of TC and TG were reduced by 11.48%~32.79% and 10.50%~18.30%, respectively; intracellular levels of TBARS were reduced by 40.33%~60.10%. These inhibitory effect of loganin treatment groups on cellular steatosis was dose-dependent and better than that of spontaneous recovery group. The results indicated that loganin could inhibit FBS-induced cellular steatosis in L02 cells via effectively reducing lipid metabolic disorders and lipid peroxidation in L02 cells.

广东省普通高校青年创新人才项目（2017KQNCX126）；岭南师范学院人才专项项目（ZL1803）