植物乳杆菌HG20对大鼠类风湿关节炎的改善作用

Improvement of Rheumatoid Arthritis with Lactobacillus plantarum HG20 in Rats

摘要:

为了获得一株对类风湿性关节炎具有辅助治疗作用的乳酸菌新菌株，本研究采用形态学观察、API50 CHL糖发酵实验、16S rDNA和pheS基因序列比对，对一株分离自黄瓜表面的乳杆菌新菌株HG20进行鉴定。通过踝关节肿胀程度、血清炎症因子水平和类风湿相关指标，评价菌株HG20对Ⅱ-型胶原诱导的类风湿性关节炎模型大鼠的治疗作用。结果表明：HG20菌株被鉴定为植物乳杆菌（Lactobacillus plantarum）。动物实验结果显示，给药21 d后，HG20组大鼠踝关节肿胀周长由3.98 cm降低至1.31 cm（65.52%）；足趾容积由6.61 mL降低至2.35 mL（61.28%）。与模型组比较，植物乳杆菌HG20可显著降低血清炎症因子IL-1β（38.26%）、TNF-α（52.58%）水平，提高抗炎因子IL-10表达（55.38%），减少C反应蛋白（CRP 12.89%）、免疫球蛋白IgG（46.07%）和IgM（34.29%）水平。综上所述，植物乳杆菌HG20通过降低炎症反应和调节自身免疫，对类风湿性关节炎具有辅助治疗作用，是一株有潜在应用前景的益生菌新菌株。

Abstract:

In order to obtain a new strain of lactic acid bacteria with adjuvant therapy of rheumatoid arthritis, morphological observation, API50 CHL sugar fermentation experiment, 16S rDNA and pheS gene sequence comparison were performed to isolate a strain HG20 from the surface of cucumber for further identification. The therapeutic effect of the HG20 strain on rheumatoid arthritis model rats induced by type II collagen was evaluated according to the indices including the degree of ankle swelling, levels of serum inflammatory factor, and rheumatoid-related indicators. The results showed that HG20 was identified as a Lactobacillus plantarum strain. Animal experiment results showed that after 21 days of administration, the ankle circumference of rats in the HG20 group decreased from 3.98 cm to 1.31 cm (by 65.52%), and the toe volume decreased from 6.61 mL to 2.35 mL (by 61.28%).Compared with the model group, L. plantarum HG20 could significantly reduce the levels of serum inflammatory cytokines (IL-1β by 38.26%; TNF-α by 52.58%), increase the expression of anti-inflammatory factor (IL-10 by 55.38%),reduce C-response protein (CRP by 12.89%) and immunoglobulin (IgG by 46.07%; IgM by 34.29%). In summary, L. plantarum HG20 has adjuvant therapeutic effects on rheumatoid arthritis through reducing inflammatory response and regulating autoimmunity. It is a new probiotic strain with potential application prospects.