豌豆低聚肽对2型糖尿病小鼠肝脏PI3K/AKT/FOXO1信号通路的调节作用

Regulatory Effect of Pea Oligopeptide on PI3K/AKT/FOXO1 Signaling Pathway in Liver of Type 2 Diabetic Mice

摘要:

本文旨在探究豌豆低聚肽对2型糖尿病(T2MD)小鼠肝脏磷脂酰肌醇-3激酶/蛋白激酶B/转录因子FoxO1(PI3K/ AKT/ FOXO1)蛋白表达的影响。通过对小鼠腹腔注射链脲佐菌素(STZ)，建立2型糖尿病小鼠模型，并用二甲双胍和豌豆低聚肽分别饮食干预4周。结果表明，豌豆低聚肽组的小鼠糖尿病指征较模型组均有一定改善，且成剂量依赖性，其中高剂量组小鼠血糖降低了23.97%，体重增加了12.24%，肝脏中PI3K、AKT、FOXO1蛋白表达分别升高了278.49%、21.78%、80.41%；细胞病理学观察发现，高剂量组能调节细胞形态，改善糖原积累。因此，豌豆低聚肽可调节2型糖尿病小鼠肝脏PI3K/AKT/FOXO1信号通路，降低肝细胞损伤，改善部分2型糖尿病小鼠指标。本文为豌豆蛋白的高值化利用拓宽了思路，为豌豆低聚肽在糖尿病领域的应用提供了实践参考，补充完善了豌豆低聚肽的糖尿病理论。

Abstract:

The purpose of this study was to investigate the effect of pea oligopeptide on the protein expression of phosphatidylinositol 3-kinase/protein kinase B/FoxO1 (PI3K/ AKT/ FoxO1) in the liver of mice with type 2 diabetes mellitus (T2MD). Mice were intraperitoneally injected with streptozotocin (STZ) to establish a type 2 diabetic mouse model, and the mice were fed with metformin and pea oligopeptide for 4 weeks, respectively. The results showed that the diabetes indications of mice in the pea oligopeptide group were improved in a dose-dependent manner compared with the model group. In the high-dose group, blood glucose was decreased by 23.97%, body weight were increased by 12.24%, and the expressions of PI3K, AKT and FOXO1 proteins in the liver were increased by 278.49%, 21.78% and 80.41%, respectively. Cytopathological observation showed that the high dose group could regulate cell morphology and improve glycogen accumulation. Therefore, pea oligopeptidatin can regulate the PI3K/AKT/FOXO1 signaling pathway in the liver of mice with type 2 diabetes, which can not only reduce liver cell damage, but also improve some indicators of type 2 diabetic mice. This paper broadens the idea of high-value utilization of pea protein, and provides practical reference for the application of pea oligopeptide in the field of diabetes, which can supplement and improve the diabetes theory of pea oligopeptide.