通过超高效液相色谱与飞行时间质谱联用（UHPLC-Q-TOF-MS/MS）技术对阴干和冷冻干燥两种不同处理方式紫苏的化学成分进行定性分析，并探讨同一来源紫苏的两种不同处理方式成分差异的物质基础。采用高效液相色谱法条件：Welch Ultimate UHPLC C18色谱柱（2.1 mm×100 mm，1.8 μm），流动相以0.1%甲酸水溶液-乙腈梯度洗脱，质谱条件：AB Sci ex Triple TOF 5600高分辨串联质谱仪，电喷雾（ESI）离子源，在正负离子模式下采集数据，通过保留时间、精确分子离子峰，二级质谱裂解碎片及相关文献对紫苏进行成分鉴定，总共鉴定了51个成分，其中紫苏阴干、冷冻干燥品中分别鉴定了45、35个化合物（包括黄酮类，苯丙素类，有机酸类等），且有9个化合物（4个有机酸，4个黄酮类，1个苯丙素）在紫苏中未见报道。可见紫苏经过阴干和冷冻干燥处理后成分会发生变化，且紫苏阴干及冷冻干燥两种不同处理方式鉴别出成分也存在差异。该研究为紫苏不同药效需求选择不同处理方法提供参考，进一步为紫苏的深入质量评价，及体内药效物质的阐明提供有效数据。

UHPLC-Q/TOF MS was used to qualitatively analyze the chemical components of Perilla frutescens treated by shade-drying menthod or freeze-drying method, and the material basis of the differences between the two treatments from the same source was discussed. High performance liquid chromatography (HPLC) conditions were as follows: Welch Ultimate UHPLC C18 column (2.1mm X100mm, 1.8 μm), the mobile phase gradient elution with 0.1% formic acid water solution and acetonitrile. The mass spectrometry conditions were as follows: AB Sci ex Triple TOF 5600 high resolution tandem mass spectrometry, electrospray ionization (ESI) ion source, data collection in positive and negative mode, fragmentation by retention time, accurate molecular ion peak, and two stage mass spectrometry. A total of 51 compounds were detected in Perilla frutescens, 45 compounds in shade-drying Perilla frutescens, 35 compounds in freeze-drying Perilla frutescen (including flavonoids, styrene acrylic element class, organic acids, etc.). There are nine compounds (four organic acids, four flavonoids, one element of styrene acrylic) was the first identified in Perilla. The composition could be changed after Perilla treated with the shade-drying or freeze-drying. The chemical components were different between Perilla treated with shade-drying method and Perilla treated with freeze-drying method. The results were helpful to choose different treatment methods of Perilla for certain medicinal value. It could be also helpful in further evaluation for Perilla, and their medicinal substances in the body.

江西省卫健委中医药科研项目（2016A045）