上转换发光纳米技术检测食品中磺胺类药物含量

Detection Sulfonamide in Food by Upconversion Fluorescence Nanotechnology

摘要:

本文基于上转换发光纳米技术建立了一种灵敏、稳定的检测食品中含五元杂环磺胺类药物的方法。利用上转换发光纳米材料作为能量供体，金纳米颗粒作为能量受体，构建荧光共振能量转移体系，通过荧光量的恢复量来定量游离抗原以达到快速检测含五元杂环磺胺类药物。结果表明，与抗体相偶联的上转换发光纳米材料和包被抗原的金纳米颗粒能够很好地结合在一起，具有良好的灵敏度和特异度。荧光的恢复量与磺胺类抗原浓度(范围在0.08~100 ng/mL)呈现出良好的线性关系，相关系数为0.99371，可以检测到磺胺类药物的最低检出限为0.08 ng/mL。同时，该方法成功应用于牛奶中，能够识别出含五元杂环磺胺类的药物，且操作简单、特异性好。基于上转换发光纳米技术构建荧光共振能量转移体系检测含五元杂环磺胺类药物残留的方法可为食品中兽药残留的检测提供新思路。

Abstract:

In this study, a sensitive and stable method for detecting five-membered heterocyclic sulfonamides in foods was established based on up-conversion fluorescent nanoparticles technology. Using upconverting nanoparticles as energy donors and gold nanoparticles as energy acceptors, a fluorescence resonance energy transfer system was constructed, and the free antigen was quantified by the amount of recovered fluorescence to achieve rapid detection of five-membered heterocyclic sulfonamides. The results showed that the upconverting luminescent nanomaterials conjugated with the antibody and the gold nanoparticles coated with the antigen can be well combined with good sensitivity and specificity. The fluorescence quenching of the upconversion fluorescent nanoparticles was linearly correlated with the concentration of the sulfonamide antigens (in the range of 0.08~100 ng/mL) present in the system, the correlation coefficient was 0.99371, enabling quantification of the sulfonamides and the lowest detection limit was 0.08 ng/mL. At the same time, this method has been successfully applied to milk, and can identify drugs containing five-membered heterocyclic sulfonamides, with simple operation and good specificity. The method of constructing a fluorescence resonance energy transfer system based on upconversion fluorescent nanoparticles technology can provide new ideas to detect residues of five-membered heterocyclic sulfonamides in food.