超声波辅助-绿色低共熔溶剂提取茉莉花黄酮的工艺优化

Optimization of Ultrasonic Assisted-Green Deep Eutectic Solvent Extraction of Flavonoids from Jasmine

摘要:

本文以茉莉花为原料，通过比对水、乙醇和8种低共熔溶剂在内的10种溶剂，以及超声波和微波提取，旨在探索一种绿色、高效的茉莉花黄酮提取技术。通过单因素试验考察低共熔溶剂的摩尔比、含水量、液料比、超声时间以及超声功率对黄酮得率的影响。在单因素的基础上，采用响应面法对提取工艺进行优化。研究表明：茉莉花黄酮最优提取技术为超声波辅助-绿色低共熔溶剂提取；最佳工艺条件为：甜菜碱盐酸盐:蔗糖:水=1:1:94.50，液料比为149:1 (mL/g)，时间为20 min，超声功率为90 W，此条件下提取量为15.24 mg/g，实验结果与响应面模型预测值接近，表明模型适用。茉莉花黄酮经此工艺所得的提取量是传统水提的677%、正交优化超声波提取的200%，充分表明超声波辅助-绿色低共熔溶剂提取技术的高效性。研究结果为茉莉花资源的进一步开发利用提供数据支撑。

Abstract:

Ten extract solvents including water, ethanol and eight types of deep eutectic solvents with microwave-assisted extraction and ultrasonic -assisted extraction were studied in order to explore a green and efficient technology for extraction of total flavonoids from jasmine. The effects of molar ratio of deep eutectic Solvents, water content, liquid - material ratio, ultrasonic time and ultrasonic power on the yield of flavonoids were investigated by single factor test. Based on single-factor tests, the quadratic regression parasitism model was established for obtain the optimal extraction conditions of flavonoids from Jasmine through the response surface methodology. It was found that the optimal extract process was ultrasonic assisted-green deep eutectic solvent, and extraction conditions were betaine hydrochloride/sucrose/H2O (1:1:94.50), liquid-material ratio 149:1 mL/g, time 20 min, ultrasonic power 90 W, the maximum yield of total flavonoids reached 15.24 mg/g, which is 677% of water extraction and 200% of orthogonal design-ultrasonic extraction. Response surface methodology was proved to be applicable for optimization of ultrasonic assisted-green deep eutectic solvent extraction of total flavonoids from Jasmine. Data support was provided for the further development and utilization of Jasmine resources.