人参皂苷F2具有抗癌、护肝、抗炎、降脂等多种生理功能，但是其关于减肥的作用机制尚不明确。本研究利用网络药理学方法预测人参皂苷F2减肥作用的潜在靶点和作用通路，借助PubChem、GeneCards数据库收集人参皂苷F2、肥胖靶点信息，通过共享靶点，利用Cytoscape等平台构建并分析人参皂苷F2-靶点-肥胖网络。结果表明，人参皂苷F2减肥作用共涉及35个潜在靶点，其中关键靶点包括MAPK1、VEGFA、CASP3等，GO富集提示潜在靶点主要参与代谢、结合、组成细胞组分等生物学过程，通过EGFR抗酪氨酸激酶抑制剂信号通路、P13K-Akt信号通路、神经活性配体-受体相互作用信号通路等方式发挥减肥作用。网络药理学成功预测了人参皂苷F2减肥作用的多靶点、多通路模式，为后续深入探讨其作用机制提供理论依据和研究方向。

Ginsenoside F2 has various physiological functions, such as anti-cancer, liver protection, anti-inflammatory, and lipid-lowing. However, its mechanism of improving obesity is still unclear. Hence, the potential targets and mechanisms of ginsenoside F2 action for anti-obesity were analyzed by network pharmacology in this study. Targets information of ginsenoside F2 and obesity was collected from PubChem and GeneCards databases. After sharing targets, the G-F2-targets-obesity network was constructed and analyzed by means of CytoScape and other platform. The anti-obesity of ginsenoside F2 was related to a total of 35 potential targets, of which included MAPK1, VEGFA, CASP3, etc. GO enrichment suggested that potential targets were mainly involved in metabolism process, binding and cellular component formation. Moreover, it may play an anti-obesity effect through EGFR tyrosine kinase inhibitor resistance, PI3K-Akt signaling pathway and neuroactive ligand-receptor interaction pathway. Network pharmacology successfully predicted the multi-targets and multi-pathways of ginsenoside F2, and it may provide a theoretical basis for further exploration of anti-obesity of ginsenoside F2.

新疆农业大学研究生科研创新项目（XJAUGRI2020018）