负载小麦醇溶蛋白纳米银胶体颗粒的抗菌壳聚糖复合膜构建及表征

Development and Characterization of Antibacterial Chitosan Composite Films Incorporated with Gliadin-coated AgNPs

摘要:

本研究采用浇筑法，成功构建了Ag NPs/小麦醇溶蛋白/壳聚糖复合抑菌膜，实现了Ag NPs的稳态化和协同增效。自组装的小麦醇溶蛋白（gliadin），同时充当Ag NPs的螯合剂和稳定剂，有效抑制了纳米银颗粒（Ag NPs）团聚。与其他复合膜相比，gliadin/AgNPs复合膜透明度良好。SEM和EDS证实Ag NPs均匀分布于复合膜。此外，gliadin /AgNPs复合颗粒具有优异的缓释特性。人皮肤成纤维细胞评估结果表明复合膜具有更好的生物相容性，激光共聚焦显微镜(CLSM)显示gliadin /AgNPs复合膜对大肠杆菌与金黄色葡萄球菌具有更强的抗菌效果。这些薄膜在抗菌食品包装材料、伤口敷料以及植入物的开发方面极具应用价值。

Abstract:

In this work, the Ag nanoparticles / gliadin / chitosan composite antimicrobial films were successfully constructed using the cast method to achieve the stabilization and synergistic effect of Ag nanoparticles (AgNPs). Self-assembled gliadin colloidal particles acted as both chelator and stabilizer of AgNPs, effectively inhibited the aggregation of AgNPs. As compared with other films, gliadin /AgNPs composite films possessed excellent transparency. SEM and EDS confirmed that AgNPs were evenly distributed on the composite film. Moreover, gliadin /AgNPs composite films had excellent sustained-release properties. Human skin fibroblast evaluation results showed that the composite films have better biocompatibility. More important, confocal laser scanning microscope (CLSM) revealed that the gliadin /AgNPs composite films possessed stronger antibacterial effect on E. coli and S. aureus. These films are of great value in the development of antibacterial food packaging materials, wound dressings and implants.