富含γ-氨基丁酸酸奶对小鼠睡眠的促进作用

Study on the Effect of High γ-aminobutyric Acid-enriched Yoghurt on Sleep and Its Mechanism on Insomnia in Mice

摘要:

采用ICR小鼠体内实验探讨富含GABA酸奶对小鼠睡眠的促进作用及机制。通过戊巴比妥钠阈下剂量催眠实验、延长戊巴比妥钠睡眠时长实验、巴比妥钠睡眠潜伏期实验研究富含GABA酸奶对小鼠睡眠的改善作用，通过测定小鼠血清和脑组织中的Gly、GABA、5-HT和Glu的含量变化探讨富含GABA酸奶对PCPA所致小鼠失眠模型的改善机制。结果显示，与阴性对照组比，富含GABA酸奶80 mg/kg、160 mg/kg剂量组小鼠入睡率均由0显著提高至60%（p<0.05）；富含GABA酸奶（80 mg/kg）组能将小鼠睡眠时长由43.40 min显著延长至156.20 min（p<0.05）；但富含GABA酸奶各剂量组均无缩短小鼠睡眠潜伏期的作用。在失眠实验中，与失眠模型组比，富含GABA酸奶能够显著增加失眠小鼠脑组织中抑制性氨基酸GABA和Gly的含量（p<0.05），GABA含量升至1.10 ng/mL，Gly含量升至28.78 ng/mL。本研究结果显示，富含GABA酸奶能够有效延长ICR小鼠的睡眠时长，改善小鼠的失眠状态，其机制与增加小鼠脑内抑制性递质GABA及Gly的含量有关。

Abstract:

The effect of high γ-aminobutyric acid-enriched yoghurt on sleep and its mechanism on insomnia was accessed by male ICR mice. Hypnosis test of pentobarbital sodium subthreshold, test of extended pentobarbital sodium sleep time and barbital sodium sleep latency test were conducted to determine the effect of high γ-aminobutyric acid-enriched yoghurt on sleep. PCPA insomnia model was used to explore the influence of high γ-aminobutyric acid-enriched yoghurt on insomnia. The changes of Gly, GABA, 5-HT and Glu in mice brain tissue and serum were measured to determine the mechanism. Compared with control group, 80 and 160 mg/kg high γ-aminobutyric acid-enriched yoghurt could improve 60% sleep rate of mice (p<0.05). 80 mg/kg high γ-aminobutyric acid-enriched yoghurt could extent mice sleep time to 156.20 min (p<0.05). 40, 80 and 160 mg/kg high γ-aminobutyric acid-enriched yoghurt did not affect mice sleep latency. In insomnia experiment, high γ-aminobutyric acid-enriched yoghurt increased the content of GABA and Gly in the brain tissue to 1.10 ng/mL and 28.78 ng/mL, respectively. In conclusion, high γ-aminobutyric acid-enriched yoghurt could improve sleep mainly by affecting the content of GABA and Gly in mice brain.