辣木油脂对多糖诱导小鼠肠道炎症的改善作用

Improvement Effects of Moringa oleracea Seed Oil on Lipopolysaccharide-induced Intestinal Inflammation in Mice

摘要:

探讨辣木油干预对LPS诱导雄性ICR小鼠肠道炎症的改善作用及机制。采用脂多糖（LPS，3.5 mg/kg）腹腔注射建立小鼠肠道炎症模型后，连续7 d给予辣木油灌服，玉米油为对照。观察小鼠体重、结肠组织变化情况。酶联免疫吸附测定法（ELISA）分别测定血清及结肠中细胞因子[IL-1β，IL-6，IL-18，肿瘤坏死因子-α（TNF-α）]水平。qRT-PCR法检测结肠内NOD样受体蛋白3(Nlrp3)，凋亡相关斑点样蛋白（Asc），半胱氨酸蛋白酶-1（caspase-1）和白介素（Interleukin, IL）-1β的mRNA水平。辣木油显著抑制LPS造成的小鼠体重下降，降低小鼠结肠重量与长度比（p<0.05）。辣木油干预同时也显著降低模型小鼠血清炎性因子（IL-1β：175.44 ng/L，IL-6：82.12 ng/L，IL-18：82.91 ng/L和TNF-α：51.75 ng/L）和结肠中（IL-1β：184.12 ng/L，IL-6：58.19 ng/L，IL-18：52.32 ng/L和TNF-α：81.31 ng/L）水平，且能够显著降低模型小鼠结肠中髓过氧化物酶（MPO）水平至86.32 ng/L（vs 损伤组：113.59 ng/L，p<0.05）。与损伤组相比，辣木油分别抑制Nlrp3（39.31%），Asc（42.81%），caspase-1（35.59%）和IL-1β（28.62%）的mRNA表达。结论提示，辣木油具有改善LPS所致小鼠肠道炎症的效果。而这一机制可能与辣木油能够抑制结肠组织内NLRP3小体的过度活化有关。

Abstract:

To investigate the effect and mechanism of Moringa oleracea seed oil on LPS-induced intestinal inflammation in male ICR mice, lipopolysaccharide (LPS, 3.5 mg/kg) was injected intraperitoneally to establish the intestinal inflammation model of mice. After 7 days, Moringa oleracea seed oil was administrated by gavage, and corn oil was used as the control. Changes in the body weight and colon tissue of mice were recorded. The levels of cytokines, includingIL-1β, IL-6, IL-18 and TNF-αin serum and colon were measured by ELISA assay, respectively. The mRNA levels of Nlrp3, Asc, Caspase-1 and IL-1β in colon were detected by qRT-PCR assay. Moringa oleracea seed oil significantly inhibited the weight loss and reduced the ratio of colon weight to length in LPS-treated mice (p<0.05). At the same time, the levels of IL-1β (175.44 ng/L and 184.12 ng/L), IL-6(82.12 ng/L and 58.19 ng/L), IL-18(82.91 ng/L and 52.32 ng/L) and TNF-α (51.75 ng/L and 81.31 ng/L) in serum and colon of the model mice were significantly reduced by the intervention of Moringa oleracea seed oil. The level of MPO in colon of model mice was significantly reduced to 86.32 ng/L (vs injury group: 113.59 ng/L, p<0.05). Compared with the injured group, Moringa oleracea seed oil inhibited the mRNA expression of Nlrp3 (39.31%), Asc (42.81%), Caspase-1 (35.59%) and IL-1β (28.62%). These results suggestted that Moringa oleracea seed oil could improve the intestinal inflammation in LPS-treated mice. The mechanism might be due to the reducing the activation of NLRP3 by Moringa oleracea seed oil in LPS-treated mice.