潮州市市售豆制品的转基因成分检测

Transgenic Analysis of Soybean Products Sold in Chaozhou City

摘要:

为了解潮州市区市售豆制品的转基因成分状况，随机购买潮州市区主要市场市售豆制品进行检测与分析。用CTAB法即改良十六烷基三甲基溴化铵，对豆制品的DNA成分进行提取并对提取到的豆制品DNA用核酸蛋白分析仪测量OD值，利用核酸定性聚合酶链式反应（Polymerase Chain Reaction，PCR）、实时定量PCR等技术对外源基因CP4-EPSPS、NOS和CaMV35S进行检测。对潮州市区主要市场市售豆制品29份样品进行检测，研究结果表明，提取到的豆制品DNA溶液浓度和纯度都较高，OD260 nm/OD280 nm介于1.9~2.1之间，抽取到的DNA质量浓度达到200 ng/μL以上，能够满足检测要求。在检测的豆制品中，有超过一半含有转基因成分。除了大润发超市外，豆制品没有任何食品标签，所有豆制品也没有是否含有转基因成分的标签。转基因食品标识制度的实施仍处于一个初始阶段，国家应该建立起强有力的市场监管机制。因此，我们必须高度重视转基因食品标签制度落实及其相应的市场监管工作。

Abstract:

In order to understand the condition of genetically modified soybean products on the market in Chaozhou City, the soybean products in the main markets were randomly purchased for testing. The DNA components of soybean products were extracted by modified CTAB method, and OD values were measured by nucleic acid protein analyzer. The exogenous genes CP4-EPSPS, NOS and CaMV35S were detected by qualitative and real-time quantitative PCR. A total of 29 soybean products sold in the main markets of Chaozhou City were investigated and tested. The results showed that the concentration and purity of DNA extracted from soybean products were higher, OD260 nm/OD280 nm ranged from 1.9 to 2.1, and the concentration of extracted DNA reached more than 200 ng/μL, which could basically meet the test requirements. More than half of the tested soybean products contain genetically modified ingredients. Except for Darunfa Supermarket’s soybean products, there are no food labels for soybean products, and whether GMF labels are required for all soybean products or not. The implementation of GMF labeling system is still in the initial stage, and a strong market supervision mechanism should be established in China. Therefore, we must attach great importance to the implementation of the GMF labeling system and the corresponding market supervision.