贵州蓝莓蜜的成分分析及其抗氧化活性

Composition Analysis and Antioxidant Activity of Blueberry Honey from Guizhou

摘要:

以贵州蓝莓蜜为研究对象，对其理化指标、抗氧化活性、多酚成分进行研究。结果显示，贵州蓝莓蜜酸度值44.69 mmol/kg，总糖含量79.30%，葡萄糖含量（40.88%）高于果糖含量（38.41%）；总酚酸和总黄酮含量分别为20.27~24.72 mg GAE/100 g和3.88~6.77 mg RE/100 g，DPPH和ABTS自由基半抑制率分别为119.04~136.56 mg/mL和140.56~212.34 mg/mL；蓝莓蜜含15种多酚类物质（原儿茶酸、对羟基苯甲酸、香草酸、咖啡酸、对香豆酸、芦丁、阿魏酸、杨梅酮、木犀草素、桑色素、槲皮素、芹菜素、山奈酚、异鼠李素、高良姜素），其中，黄酮类物质含量最高为槲皮素，酚酸含量由高到低为：对羟基苯甲酸>对香豆酸>阿魏酸>咖啡酸>香草酸>原儿茶酸。研究表明，蓝莓蜜酸度偏高、易结晶，结合槲皮素含量高的特点与酚酸含量高低规律，可作为蓝莓蜜鉴定与检测的参考指标。

Abstract:

Taking the blueberry honey of Guizhou as the research object, its physicochemical indices, antioxidant activity, and polyphenol composition of blueberry honey of Guizhou were analyzed. The results showed that blueberry honey of Guizhou had an acid value of 44.69 mmol / kg, total sugar content of 79.30%, with its glucose content (40.88%) higher than its fructose content (38.41%). The total phenolic acid and total flavonoid contents were 20.27~24.72 mg GAE/100 g and 3.88~6.77 mg RE/100 g, respectively. The IC50 values for the DPPH free radical and ABTS free radical scavenging rates were 119.04~136.56 mg/mL and 140.56~212.34 mg/mL, respectively. The blueberry honey contains a total of 15 polyphenolic compounds (protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid, rutin, ferulic acid, myricetin, uteolin, morin, quercetin, apigenin, kaempferol, isorhamnetin, galangin). Quercetin had the highest content among flavonoids, with the content of individual phenolic acid decreasing in the order of p-hydroxybenzoic acid>p-coumaric acid>ferulic acid>caffeic acid>vanillic acid>protocatechuic acid. The research showed that blueberry honey had a relatively high acidity and tended to crystallize. The high quercetin content along with the changing pattern of phenolic acid content can be used as the reference indices for blueberry honey identification and detection.