不同产地马齿苋的抗衰老活性比较

Comparison of Anti-aging Effects of Portulaca oleracea L. from Different Areas

摘要:

为了研究不同产地马齿苋的化学成分和活性差异，本文选取了四个不同产地的马齿苋作为样品，利用福林酚比色法、硼氢化钠-四氯代苯对醌法、氧自由基吸收能力ORAC法测定了其总酚含量、总黄酮含量和其抗氧化值，并通过秀丽隐杆线虫模型研究样品对线虫寿命的延长效果。结果表明，四川产地的马齿苋在四个指标上都具有最高的效果：总酚含量99.28 μg GAE/mg DW，总黄酮含量121.57 μg CE/mg DW，ORAC值3.32 μmol Trolox/mg DW，能够把线虫的平均寿命从19.53 d延长到26.65 d，延长了36.49%。该延长效果具有浓度依赖性。通过相关性分析，ORAC与总黄酮含量具有强相关性（R2=0.99），而马齿苋醇提物的抗氧化活性可作为影响其其延长线虫寿命效果的指标（R2=0.95）。综上所述，马齿苋具有良好的抗衰老作用，而四川产地的马齿苋的效果最好，具有较大的发展潜力与空间。马齿苋醇提物在线虫模型中发挥抗衰老作用的过程中，很可能是其中黄酮物质起到抗氧化作用从而促进的。

Abstract:

To study the differences in chemical compositions and activities of Portulaca oleracea L. (PO) from different areas, four PO from different areas were selected as samples. The Folin-Ciocalteu, SBC and ORAC method were used to determine the total phenol content, total flavonoid content and the antioxidant value, and the effect of the sample on the life extension of nematodes was studied by C. elegans model. The results shows that PO Sichuan has the highest effect based on four indicators: total phenol content 99.28 μg GAE/mg DW, total flavonoid content 121.57 μg CE/mg DW, ORAC value 3.32 μmol Trolox/mg DW, it can prolong the mean lifespan from 19.53 days to 26.65 days, with an increase of 36.49%. The longevity effect is in a dose-dependent manner. Through correlation analysis, ORAC has a strong correlation with total flavonoid content (R2=0.99), and the antioxidant activity of the PO alcohol extract can be used as an index affecting its effect on prolonging the life of nematodes (R2=0.95). In summary, PO has good anti-aging effects, while PO from Sichuan has the best effect, and has great development potential. The anti-aging effect that alcohol extracts from PO acts in the nematode model is probably promoted by the sample antioxidant activity, which has high correlation with the flavonoid content.