本文研究了β-细辛醚快速抗抑郁作用，并对其不同给药方式的作用效果及潜在的生物学机制进行探讨。将70只昆明种雄性小鼠随机分为模型组（n=60）、正常组（n=10），模型组给予慢性不可预见性刺激，正常组常规饲养；21 d后，采用糖水偏好实验选取40只造模成功小鼠，随机分成五组：β-细辛醚鼻腔组、β-细辛醚灌胃组、越鞠丸鼻腔组、越鞠丸灌胃组、模型对照组；正常组中随机选取8只小鼠作为正常对照组。单次给药30 min后依次进行旷场实验，悬尾实验和强迫游泳实验；采用Western blot实验检测给药后45 min小鼠海马区脑源性神经营养因子（BDNF）和酪氨酸激酶B（TrkB）蛋白的表达变化。β-细辛醚用药组的旷场实验得分高于模型对照组(p<0.05)；强迫游泳实验和悬尾实验中小鼠的静止时间更短(p<0.05)；与模型对照组比较，β-细辛醚用药组给药45 min后小鼠海马中的BDNF及其受体TrkB的表达均明显上调(p<0.05)。β细辛醚具有快速抗抑郁作用，且这种潜在的快速抗抑郁作用可能与BDNF及其受体TrkB的激活相关。

The potential rapid antidepressant effect of β-asarone was investigated in this work. The effect of different administration methods of β-asarone on rapid antidepressant action and their biological mechanism were further evaluated. The 70 male KM mice were randomly divided to model group (n=60) and normal group (n=10). The model group was given chronic unpredictable stimulation, while the normal group was fed normally. After twenty-one days of feed, sucrose preference test was conducted on the model group to select 40 mice, which were successfully depression modeled. These 40 mice randomly were divided into five groups: β-asarone nasal cavity administration, β-asarone intragastric administration, Yueju pill nasal cavity administration, Yueju pill intragastric administration and model control group. Eight mice in the normal group were selected as normal control group. After 30 min of a single drug administration, open field experiment, tail suspension experiment and forced swimming experiment were conducted. Western blot was used to detect the expression of brain derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) in hippocampus of KM mice at 45 min post the single administration. The open-field test scores of the β-asarone group were higher than those of the model control group (p<0.05), forced swimming test and tail suspension test (p<0.05). Compared with the model control group, the expression of BDNF and TrkB in hippocampus of mice in the β-asarone group increased significantly after 45 minutes of administration (p<0.05). β-asarone exhibits a rapid antidepressant-like potential effect, and the potential rapid antidepressant effects might be associated with the up-regulation of BDNF and its receptor TrkB.

国家级大学生创新创业训练项目（201810632060）