Abstract:To investigate the protective effects of total extract of Panacis japonici Rhizoma on lipopolysaccharide-induced inflammation in BV2 cells and its underlying mechanisms. The viability of BV2 cells was detected by MTT. Nitric oxide (NO) levels were measured by Griess. The mRNA expression of TNF-α, IL-1β and iNOS were evaluated by PCR. Immunofluorescence was performed to detect the expression and location of NF-κB. MTT results showed that different concentrations of total extract of Panacis japonici Rhizoma (3.125~50 mg/L) have no effect on the growth of microglia, regardless of normal state or LPS(1 μg/L)-induced inflammatory condition. Compared with the normal group, the release of NO in the model group was 11.82 times, and the mRNA expressions of TNF-α, IL-1β and iNOS in the model group were 1.35 times, 1.78 times and 2.50 times, respectively. There was an obvious nuclear shift of NF-κB after LPS induction. After administration of total extract of Panacis japonici Rhizoma (3.125~50 mg/L), over-expression of IL-1β, TNF-α, iNOS and NO induced by LPS were effectively reversed. Moreover, total extract of Panacis japonici Rhizoma (25, 50 mg/L) can effectively inhibit nuclear translocation of NF-κB. Total extract of Panacis japonici Rhizoma can effectively improve LPS-induced inflammatory response in BV2 microglia. The underlying mechanism is mainly to inhibit nuclear translocation of NF-κB and thereby reducing the secretion of inflammatory factors.
