茯苓水提物对幽门螺杆菌的抑制作用和GES-1细胞增殖作用研究

Effects of Water Extract of Poria on Helicobacter pylori Inhibition and GES-1 Cells Proliferation

摘要:

本文从不同中药水提物[白术、茯苓、陈皮、山药、复合多糖（白术、茯苓、陈皮、山药的混合水提物）]中筛选具有良好抑制幽门螺杆菌（Helicobacter pylori，Hp）活性以及能促进人胃粘膜上皮（Human gastric epithelial cell line，GES-1）细胞增殖的物质。对比不同水提物对Hp的抑菌活性，体外抑制脲酶的活性。结果显示，茯苓、复合多糖及白术水提物，具有一定程度的抑制Hp作用，在浓度为1000.00 μg/mL时对Hp的抑制率分别为93.29%、62.85%、47.14%。其中，以茯苓水提物的抑菌效果最显著。各种中药水提物都具有不同程度的抑制脲酶活性，茯苓水提物的效果最好（IC50=618.90 μg/mL）。茯苓水提物对Hp的抑制呈现一定程度剂量依赖效应。当其浓度达到15.62 μg/mL以上时，对Hp的抑制率达80%以上。以125.00 μg/mL的茯苓水提物作用24 h后，即可显著地提高胃粘膜上皮细胞（GES-1）的存活率至128.35%。因此，茯苓水提物可能通过抑制幽门螺杆菌增殖及其毒力因子脲酶的活性，同时促进GES-1细胞增殖来降低幽门螺杆菌感染胃部所引起的毒性作用。

Abstract:

Inhibitory activity on Helicobacter pylori (Hp) of different water extracts of Chinese herb (Atractylodes rhizome, Poria, dried orange peel, Yam and polysaccharide complex (mixed water extracts of Atractylodes rhizome, Poria, dried orange peel and yam) was studied. The antibacterial experiment results showed that the water extract of Poria, polysaccharide complex and Atractylodes rhizome demonstrated a certain inhibitory activity on Hp. The inhibition rates were 93.29%, 62.85% and 47.14% at the concentration of 1000.00 μg/mL, respectively. The water extract of Poria showed the most significant effect. The water extract of Herb had different inhibitory effects on urease activity, and the water extract of Poria had the best effect (IC50=618.90 μg/mL). The water extract of Poria showed a dose-dependent effect on the inhibition of Hp. The inhibition rate was over 80% when the concentration reached 15.62 μg/mL or more. The survival rate of human gastric epithelial cells (GES-1) was significantly increased to 128.35% after treatment with 125.00 μg/mL of the water extract from Poria for 24 h. Therefore, the water extract of Poria might repair the Hp-injured stomach by inhibiting Hp proliferation and urease activity and promoting the proliferation of GES-1 cells.