咖啡酸苯乙酯对糖尿病小鼠心脏的保护作用

Protective Effect of Caffeic Acid Phenylethyl Ester on Heart of Diabetic Mice

摘要:

本文研究了咖啡酸苯乙酯(CAPE)对糖尿病小鼠心脏的保护作用及其作用机制。对小鼠采用皮下腹腔注射链脲佐菌素诱导及结合喂养高脂高糖饲料建立糖尿病模型。将各小鼠随机分为空白组，损伤组以及保护组。保护组用CAPE灌胃，其余2组用0.9%生理盐水灌胃，测定小鼠心脏组织内各氧化指标变化来探讨摄入CAPE对其的保护。建模成功后，损伤组超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)的含量依次降低0.00058 U/mg protein、0.0033 mg/mL tissue、20.95 U/mg protein；而给予CAPE保护后，保护组丙二醛(MDA)、蛋白羰基化程度(PCO)、一氧化氮(NO)的含量依次降低0.126 mmol/mg protein、3.205 mmol/mg protein、0.5469 μmol/mg protein，说明高糖环境可加剧心脏发生氧化应激反应，诱导产生氧化损伤；而摄入CAPE后能降低各指标含量，减弱损伤程度。氧化应激增强可能发生糖尿病，摄入CAPE后对小鼠损伤程度减弱，抗氧活性增强，自由基清除能力得到提高，从而对其心脏起到保护作用。

Abstract:

The protective effect of caffeic acid phenylethyl ester (CAPE) on the heart of diabetic mice and its mechanism were investigated. Diabetes mellitus model was established in mice by subcutaneous intraperitoneal injection of streptozotocin and feeding high-fat and high-sugar diet. The mice were randomly divided into blank group, injury group and protection group. The protective group was given CAPE by gastric perfusion, the other two groups were given 0.9% saline by gastric perfusion. The changes of oxidative indexes in heart tissue of mice were detected to evaluate the protective effect of CAPE. After successful modeling, the contents of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) in the injured group decreased by 0.00058 U/mg protein, 0.0033 mg/mL tissue and 20.95 U/mg protein in turn, respectively. The contents of malondialdehyde (MDA), protein carbonylation (PCO), nitric oxide (NO) in the protected group decreased by 0.126 mmol/mg protein, 3.205 mmol/mg protein and 0.5469 μmol/mg protein in turn after CAPE protection, respectively. Results indicated that high glucose environment could aggravate oxidative stress and induce oxidative damage in the heart, while CAPE could reduce the content of various indicators and the degree of injury. Increased oxidative stress might lead to diabetes mellitus. After CAPE treatment, the degree of injury in mice decreased, the antioxidant activity and the free radical scavenging ability were improved, resulting in their hearts potection.