Abstract:In order to improve the stability and bioavailability of 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), the inclusion compound of AA-2G in beta-cyclodextrin (β-CD) was prepared. The preparation process of AA-2G-β-CD inclusion compound was studied by saturated aqueous solution method. The effects of temperature, time, stirring speed and the molar ratio of β-CD to AA-2G on the preparation of inclusion compound were investigated by single factor experiments. The optimized conditions for the preparation of AA-2G-β-CD inclusion compound were determined by orthogonal experiments listed as follows: the molar ratio of AA-2G to β-CD as 1:3, temperature as 60 °C, the stirring speed as 200 r/min, time as 5 h, and the resulting inclusion rate was 49.55%. The order of factors affecting inclusion ratio was time>temperature>speed>molar ratio. The validation test showed that the preparation of AA-2G-β-CD inclusion compound by saturated aqueous solution method was stable. The AA-2G-β-CD inclusion compound was identified by FT-IR, proving the formation of AA-2G-β-CD inclusion compound. It was found that the free radical scavenging ability of the inclusion compound was higher than that of the AA-2G and β-CD mixture. In summary, AA-2G inclusion compound was prepared by saturated aqueous solution method. The formation of AA-2G-β-CD inclusion compound was confirmed. The inclusion rate of AA-2G-β-CD inclusion compound reached 49.55% after optimizing the preparation process by orthogonal experiments. At the same time, the antioxidant ability of the inclusion compound was higher than that of the AA-2G and β-CD mixture.
