本研究探讨紫菜薹(BCEE)、紫甘蓝(RCEE)、紫芜箐(BREE)及羽衣甘蓝(BOEE)乙醇提取物对葡聚糖硫酸钠(DSS，2%)诱发C57BL/6J小鼠溃疡性结肠炎的影响。观察小鼠体重，结肠长度，疾病活性指数及结肠组织变化。试剂盒法检测小鼠结肠髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)，过氧化氢酶(CAT)，谷胱甘肽还原酶(GSH-Px)，谷胱甘肽(GSH)和丙二醛(MDA)水平。酶联法测定肠内炎性因子(TNF-α，IL-1β，IL-6和IL-18)水平。qRT-PCR法检测结肠内炎性因子，NLRP3，ASC，caspase-1，iNOS和COX-2的mRNA水平。较模型小鼠4种紫蔬菜均显著抑制DSS致体重减轻，增加结肠长度至8.67 cm (BCEE组)，8.23 cm (BREE组)，7.66 cm (RCEE组)和7.40 cm (BOEE组)；改善肠内炎症程度；提升肠内抗氧化物酶(SOD，CAT和GSH-Px)和GSH水平；降低炎细胞因子(TNF-α、IL-1β、IL-6和IL-18)，MPO及MDA水平。此外，紫菜蔬能抑制结肠组织中炎性因子，NLRP3，ASC，caspase-1和iNOS与COX-2的mRNA表达。综上数据提示，4种紫菜蔬缓解DSS诱导小鼠结肠炎能力为BCEE>BREE>RCEE>BOEE。

The purpose of this study was to investigate the preventive effect of the ethanolic extracts from purple Brassica vegetables including Brassica campestris (BCEE), red cabbage (RCEE), Brassica rapa (BREE)and Brassica oleracea (BOEE)on dextran sulfate sodium (DSS, 2%)-induced ulcerative colitis in C57BL/6J mice. Body weight, colon length, ratio of colon length/weight, disease activity index (DAI) and histological changes were observed. Colonic myeloperoxidase (MPO), super oxygen dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione (GSH), and malondialdeyde (MDA) levels were also measured using commercial kits. Colonic levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-18 were measured with an enzyme-linked immunosorbent assay (ELISA).The colonic mRNA expressions of TNF-α, IL-1β, IL-6, IL-18, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), NLRP3, ASC and caspase-1 were quantified by a quantitative real time polymerase chain reaction (qRT-PCR) assay. Four kinds of purple Brassica vegetable extracts significantly suppressed DSS-induced body weight loss, increased the colon length (to 8.67 cm in BCEE group, 8.23 cm in BREE group, 7.66 cm in RCEE group and 7.40 cm in BOEE group), decreased colon length/weight ratio, and reduced the inflammatory reaction in colon of colitis mice. Purple Brassica vegetable extracts also reduced the levels of inflammatory cytokines (TNF-α、IL-1β、IL-6 and IL-18), MPO and MDA in the colon of colitis mice. In addition, Purple Brassica vegetable extracts also decreased the mRNA expressions of inflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-18), NLRP3, ASC,caspase-1, iNOS and COX-2.In summary, the four kinds of purple Brassica vegetable extracts were able to attenuate the DSS induced colitis in mice. The order of relieving DSS-induced colitis activity was BCEE>BREE>RCEE>BOEE.

国家自然科学基金项目(81560530、81760589)；广西自然科学基金项目(2016GXNSFCA380026)；广西高等学校千名中青年骨干教师培育计划资助项目(桂教人[2018]18号)；桂林医学院引进人才科研启动基金项目(04010150001)