金黄色葡萄球菌的耐药性及生物被膜形成能力研究

Drug Resistance and Biofilm-forming Ability of Staphylococcus Aureus

摘要:

为分析金黄色葡萄球菌的耐药情况及生物被膜形成能力，以70株金黄色葡萄球菌为研究对象。利用纸片扩散法分析其耐药谱，组合利用刚果红平板测试法和结晶紫染色法分析其生物被膜形成能力。应用PCR技术检测生物被膜形成相关基因，并分析这些菌株耐药谱与其生物被膜形成能力强弱之间的关系。结果表明：金黄色葡萄球菌菌株耐药情况较为严重，其中97.14%的菌株具有耐药性；所有菌株均能形成生物被膜，能形成强、中等与弱粘附生物被膜能力的菌株分别占71.43%、18.57%和10%；生物被膜相关基因fnbA、fnbB和clfB的检出率分别为57.14%、20.00%和44.29%；生物被膜形成能力强的菌株，其耐药谱更广，且对利奈唑胺的耐药率差异有统计学意义（p<0.05）。临床来源的金黄色葡萄球菌多重耐药性和生物被膜普遍存在，已成为食品安全中的隐患来源，研究结果为控制金黄色葡萄球菌感染提供理论依据。

Abstract:

To study the drug resistance and biofilm-forming ability of Staphylococcus aureus (S. aureus), 70 strains of S. aureus were used as research objects. Their drug resistance spectra were analyzed by the paper diffusion method, while their biofilm-forming ability was examined using the Congo red plate test and crystal violet staining method. The biofilm formation related genes were detected by PCR, and the relationship between the drug resistance spectrum of these strains and their biofilm-forming abilities was analyzed. The results showed that the drug-resistance of S. aureus strains was more severe, of which 97.14% were resistant strains. All the strains could form biofilm, and the strains capable of forming biofilms with strong, medium and weak adhesive strength accounted for 71.43%, 18.57% and 10%, respectively. The detection rates of biofilm-related genes, fnbA, fnbB and clfB, were 57.14%, 20.00% and 44.29%, respectively. The strain with strong biofilm-forming ability had a broader spectrum drug resistance, and exhibited statistically significant (p<0.05) differences in the resistance rate to linezolid. The multi-drug resistance and ubiquitous biofilm of clinically-derived S. aureus have become a source of food safety hazards. The research results provide a theoretical basis for controlling S. aureus infections.