不同方法处理的浒苔的小鼠体内抗氧化活性比较

Comparison of Antioxidation Activities in a Mouse Model for Enteromorpha Processed with Different Methods

摘要:

本研究比较了三种不同方法处理的浒苔在小鼠体内的抗氧化作用，在衰老模型小鼠灌胃给予浒苔原粉（EO），浒苔多糖(EP)，浒苔超声处理粉末（EU-L）低剂量，浒苔超声处理粉末（EU-H）高剂量分别为300 mg/kg·d，100 mg/kg·d，300 mg/kg·d，350 mg/kg·d，7周后检测其对小鼠血清、肝脏、脑组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）、过氧化氢酶（CAT）含量的影响。结果表明，浒苔产品对小鼠体重增长无影响；其中，血清中CAT含量由模型组的6.90U/mL提高到EP组8.79 U/mL，EU-L 组8.31 U/mL，EU-H组9.43 U/mL,与模型组对照差异极显著（p<0.01），EU-H组小鼠血清、肝和脑组织的SOD、GSH-Px、CAT含量均显著高于模型组（p<0.01）；给予浒苔产品的四组小鼠MDA含量均低于模型组9.94U/mL，7.88U/mg prot，6.63U/mg prot (p<0.05)。说明，浒苔超声粉高剂量组（EU-H）能够有效提高衰老模型小鼠体内抗氧化酶活性，对衰老小鼠的治愈率达90%以上，效果优于浒苔多糖（EP）；而且根据剂量比较，浒苔超声粉（EU）可以剂量依赖性地提高衰老小鼠体内抗氧化能力。

Abstract:

This study compared the antioxidant effects of three Enteromorpha products processed with different processing methods in a mouse model. Aged mice were administrated by gavage with enteromorpha original (EO, 300 mg/kg·d), enteromorpha polysaccharide (EP, 100 mg/kg·d), low dose of enteromorpha ultrasound (EU-L, 300 mg/kg·d) or high dose of enteromorpha ultrasound (EU-H, 350 mg/kg·d). The contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), catalase (CAT) in serum, liver and brain tissues of mice were analyzed after 7 weeks. The results showed that all these enteromorpha products had no effect on the weight gain of the mice. The serum CAT content in model group was 6.90 U/mL, which was significantly (p<0.01) lower than to those for the EP group (8.79 U/mL), EU-L group (8.31 U/mL) and EU-H group (9.43 U/mL). The contents of SOD, GSH-Px and CAT in serum, liver and brain tissues of the EU-H were significantly (p<0.01) higher than the model group. The MDA contents of the four groups fed with enteromorpha products were lower (p<0.05) than that of the model group：9.94 U/mL, 7.88 U/mg prot, 6.63 U/mg prot (p<0.05).These results indicate that EU-H effectively improved the activities of antioxidant enzymes in the aged mouse model with the rate of reversing aging over 90%，showing advantages over EP; According to the comparison on dose, EU could increase the antioxidant effect on aged mice in a dose-dependent manner.