[关键词]
[摘要]
本试验旨在对双峰驼血液(驼血)中营养物质含量及其血液学参数进行测定和评估。利用BCA蛋白浓度试剂盒对阿拉善双峰驼血液总蛋白含量进行测定,采用液相色谱及质谱连用分析法对其氨基酸、脂肪酸和矿物质含量进行测定,利用全自动血细胞分析仪对其血液参数进行测定。结果表明:驼血总蛋白含量为78.45%±1.49%。驼血水解后共得到34种氨基酸,其中赖氨酸含量最高(27.82±1.86 g/L),其次为精氨酸(23.60±4.17 g/L),天门冬氨酸(22.41±1.26 g/L);驼血中必需氨基酸占氨基酸总含量的41.01%,必需氨基酸与非必需氨基酸的比值为0.69,符合FAO/WHO提出的参考蛋白模式。驼血中共检测出19种脂肪酸,其中戊二酸含量最高(1900.70±571.02 μg/mL)其次为油酸(1342.00±194.12 μg/mL),二十碳五烯酸含量最低(11.22±5.24 μg/mL);驼血中饱和脂肪酸占全部脂肪酸含量的60.89%,单不饱和脂肪酸和所占比例为21.32%,多不饱和脂肪酸所占比例为17.05%,长链脂肪酸所占比例为47.50%,中链脂肪酸所占比例为52.50%,奇数碳脂肪酸所占比例为36.13%。驼血中富含人体中所需的各类矿物质,其中钠(5300±2300 mg/kg)含量最高,其余依次为钾(1600±100 mg/kg)、铁(452.00±35.75 mg/kg)、磷(300±20 mg/kg)、钙(90.71±6.03 mg/kg)、镁(39.40±2.26 mg/kg)、锌(12.43±1.17 mg/kg)、铜(0.65±0.06 mg/kg)、锰(54.78±12.32 μg/kg)、铬(10.21±0.89 μg/kg)。驼血中红细胞、白细胞及血红蛋白含量均高于牛、羊等畜种,且其免疫细胞的分布比例也与大多数畜禽不一致。
[Key word]
[Abstract]
This experiment aimed to determine and evaluate the nutrient content and hematological parameters of the blood in Bactrian camels (camel blood). The total protein content of the blood in Alashan Bactrian camel was determined using BCA protein concentration kit. The contents of amino acids, fatty acids and minerals in the blood were determined by liquid chromatography and mass spectrometry. The blood parameters were measured using an automatic blood cell analyzer. The results showed that the total protein content of camel blood was 78.45%±1.49%. There were 34 amino acids after the hydrolysis of camel blood, with the lysine content being the highest (27.82±1.86 g/L), followed by arginine (23.60±4.17 g/L) and aspartic acid (22.41±1.26 g/L). The essential amino acids in camel blood accounted for 41.01% of the total amino acids, with the ratio of essential amino acids to non-essential amino acids as 0.69, which conforms to the reference protein pattern proposed by FAO/WHO. A total of 19 fatty acids were detected in camel blood, and among which, glutaric acid had the highest content (1900.70±571.02 μg/mL), followed by oleic acid (1342.00±194.12 μg/mL) and eicosapentaenoic acid (11.22±5.24 μg/mL, the lowest). The saturated fatty acids accounted for 60.89% of all the fatty acids in camel blood, and monounsaturated and polyunsaturated fatty acids were responsible for 21.32% and 17.05%, respectively, with long chain, medium chain and odd carbon fatty acids accounting for 47.50%, 52.50%, and 36.13%, respectively. The camel blood was rich in all kinds of minerals needed by the human body, among which the content of sodium was the highest (5300±2300 mg/kg), followed by potassium (1600±100 mg/kg), iron (452.00±35.75 mg/kg), phosphorus (300±20 mg/kg), calcium (90.71±6.03 mg/kg), magnesium (39.40±2.26 mg/kg), zinc (12.43±1.17 mg/kg), copper (0.65±0.06 mg/kg), manganese (54.78±12.32 μg/kg) and chromium (10.21±0.89 μg/kg). The contents of red blood cells, white blood cells and hemoglobin in camel blood were higher than those of cattle and sheep, and the distribution of immune cells was also inconsistent with that for most livestock.
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[基金项目]
内蒙古自治区科技重大项目;双一流学科创新团队建设(NDSC2018-14);高层次人才引进科研启动项目(NDYB2017-28);内蒙古自然科学基金项目(2018BS03017);内蒙古自治区科技创新引导项目(KCMS2018048)